Purchase of a Beckman XL-1 Analytical Ultracentrifuge

职位描述(申请人提供)：来自三个校区的九名调查人员 马里兰大学为Beckman Coulter Optima XL-I寻求资金 分析性超速离心机。用户组由Beckett博士(P1)组成， 来自化学系的Davis，Isaacs，Julin，Kahn，Munoz和Greer 生物化学和化学工程与生物结构中心 UMCP&组织，生物化学系的David Weber博士和 巴尔的摩马里兰大学分子生物学和迈克尔博士 萨默斯来自哈佛大学HHMI和化学与生物化学系 马里兰州，巴尔的摩县。离心机将用于三年后的研究 一般领域包括分析体系中的大分子组装 生物兴趣、多肽组装性质的测定和 用于折叠和高分辨率结构研究的蛋白质，以及 合成仿生剂的能量学和化学计量学表征 装配。具体项目包括测定能量学和 转录调控中蛋白质-蛋白质相互作用的化学计量学 以及DNA重组和修复，模型离子通道的分析，以及 折叠研究用模型多肽组装性质的测定。 由吸收和干涉光学提供的多功能性 Beckman Optima XL-I分析超速离心机将是成功的关键 在本提案中描述的广泛应用中的一种。

DESCRIPTION (provided by applicant): Nine investigators from three campuses of the University of Maryland seek funding for a Beckman Coulter Optima XL-I analytical ultracentrifuge. The user's group consists of Drs. Beckett (P1), Davis, Isaacs, Julin, Kahn, Munoz, and Greer from the Departments of Chemistry & Biochemistry and Chemical Engineering and the Center for Biological Structure & Organization at UMCP, Dr. David Weber from the Department of Biochemistry and Molecular Biology at the University of Maryland, Baltimore and Dr. Michael Summers from the HHMI and Department of Chemistry & Biochemistry, University of Maryland, Baltimore County. The centrifuge will be used for research in three general areas including analysis of macromolecular assembly in systems of biological interest, determination of the assembly properties of peptides and proteins for folding and high resolution structural studies, and characterization of the energetics and stoichiometries of synthetic biomimetic assemblies. Specific projects include determination of the energetics and stoichiometries of protein-protein interactions in transcriptional regulation and DNA recombination and repair, analysis of model ion channels, and determination of the assembly properties of model peptides for folding studies. The versatility provided by the absorption and interference optics of the Beckman Optima XL-I analytical ultracentrifuge will be essential to the success of the broad range of applications described in this proposal.