Core--cell, tissue and animal facility

描述：(申请人提供)：该核心的目的是协助 治疗和/或辅助治疗策略的设计 预防人类免疫缺陷病毒感染后的神经系统疾病 1型(HIV-1)感染。在这方面，纯化的人类单核细胞来源 将提供巨噬细胞(MDM)和MDM条件培养液(MCM)，如下 艾滋病毒-1感染和/或免疫激活，应要求向所有调查人员提供， 在罗切斯特合作神经艾滋病药物发现小组(RCNDDG)内。在……里面 此外，开发了前景看好的抗炎和/或神经保护药物 在实验室化验中或建议中，将在 严重联合免疫缺陷(SCID)小鼠HIV-1脑炎模型 (蜂巢)。脑组织和/或血清将可用于药物检测 在蜂箱小鼠中的水平和病理学。这样的作品将支持翻译 (从工作台到床边)研究工作，并直接影响到 随后的临床试验。这些作品，总的来说，都是基于这样的概念 HIV-1相关性痴呆(HAD)部分是一种可逆的代谢 病毒感染单个核细胞免疫缺陷所致脑病 吞噬细胞[(MP)、小胶质细胞、血管周围和实质巨噬细胞]。这些 MPS既是产生HIV-1感染的宿主，也是主要的 中枢神经系统(CNS)内神经毒性活动的来源。这个 开发抑制大脑中毒性炎症活动的方法可能会 既能改善又能预防持续性病毒的并发症 脑内复制作为正在进行的关键辅助治疗 有效的抗逆转录病毒疗法，这是RCNDDG的主要目标。

DESCRIPTION: (Provided by applicant): The purpose of this core is to assist in the design of adjunctive therapeutic strategies for treatment and/or prevention of neurologic disease following human immunodeficiency virus type-one (HIV-1) infection. In this regard, purified human monocyte-derived macrophages (MDM) and MDM conditioned media (MCM) will be supplied, following HIV-1 infection and/or immune activation, to all investigators on request, within the Rochester Cooperative NeuroAIDS Drug Discovery Group (RCNDDG). In addition, promising anti-inflammatory and/or neuroprotective drugs, developed in laboratory assays or proposed, will be tested for therapeutic efficacy in a severe combined immune deficiency (SCID) mouse model of HIV-1 encephalitis (HIVE). Brain tissue and/or sera will be made available for measuring drug levels and pathology in the HIVE mice. Such works will support translational (bench to bedside) research efforts and directly effect the performance of subsequent clinical trials. The works, in toto, are based on the concept that HIV-1 associated dementia (HAD) is, in part, a reversible metabolic encephalopathy caused by defective immunity of virus-infected mononuclear phagocytes [(MP), microglia, perivascular and parenchymal macrophages]. These MPs serve both as reservoirs for productive HIV-1 infection and principal sources of neurotoxic activities within the central nervous system (CNS). The development of ways to inhibit toxic inflammatory activities in brain may serve to both ameliorate and prevent complications of persistent viral replication in brain serving as critical adjunctive therapies to ongoing potent anti-retroviral regimens, the principal goals of the RCNDDG.