HIGH RESOLUTION DATA ON DIFFERENT CDK2 CKS COMPLEXES

CKS细胞周期调节蛋白（CksHs 1和CksHs 2） 人，裂殖酵母中的suc 1和芽殖酵母中的CKS 1）与 细胞周期蛋白依赖性激酶的催化亚基（Cdks，如CDK 2或CDK 3）， CDC 2），并且是体内细胞周期进展所必需的。 我们有 我最近将CDK 2-CksHs 1复合物结晶成两种不同的晶体 forms. 在更高分辨率下的这种复合物的结构是至关重要的 了解Cks函数。 由于CDK 2结合人CKS或 酵母同源物suc 1或CKS 1，我们还计划用一个 的酵母CKS蛋白质，以证明这些优良的特异性， 蛋白质对Cdks。

The CKS cell cycle regulatory proteins (CksHs1 and CksHs2 in human, suc1 in fission yeast and CKS1 in budding yeast) bind to the catalytic subunit of the cyclin-dependant kinases (Cdks as CDK2 or cdc2) and are essential for cell cycle progression in vivo. We have recently crystallized the CDK2-CksHs1 complex in two different crystal forms. A structure of this complex at higher resolution is critical to understand the Cks function. As CDK2 binds either human CKS or yeast homologs suc1 or CKS1, we also plan to crystallize CDK2 with one of the yeast CKS proteins to demonstrate the fine specificity of these proteins towards Cdks.