STRUCTURE OF COMPLEX BETWEEN TFIIIA & ITS 31 BP DNA SITE
STRUCTURE OF COMPLEX BETWEEN TFIIIA & ITS 31 BP DNA SITE
批准号:
6658592
负责人:
STEPHEN COPLAN HARRISON
金额:
$14.32万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2002
资助国家:
美国
项目状态:
已结题
起止时间:
2002-03-01 至 2003-02-28
中文摘要
最初在非洲爪蟾中发现了多个锌指基序
转录因子IIIA(TFIIIA)。 每个主题都是独立的
折叠蛋白质结构域。 九个串联的锌指存在于
TFIIIA,允许蛋白质结合到5S
RNA基因启动子。 有一个相对“标准”的对接大多数
在DNA的大沟中,
GLI结构显示一个指状物基本上没有DNA接触。
因此,具有多于3个指状物的蛋白质可以具有高度趋异性。
对接模式。 本研究的目的是检查一个仍然
更长的复杂。
英文摘要
Multiple zinc finger motifs were originally discovered in Xenopus
transcription factor IIIA (TFIIIA). Each motif is an independently
folded protein domain. Nine tandem zinc fingers are present in
TFIIIA, allowing the protein to bind to an extensive region of the 5S
RNA gene promoter. There is a relatively "standard" docking of most
of the fingers represented in set into the major groove of DNA, but
the GLI structure shows one finger with essentially no DNA contacts.
Thus, proteins with more than 3 fingers may have highly divergent
docking modes. The goal of the present study is to examine a still
longer complex.
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