CELL ENTRY AND SPREAD BY POLYOMA VIRUS

本项目的总体目标是更好地了解 多瘤病毒进入和传播细胞的机制。这种病毒会感染和 在培养中转化啮齿动物细胞。当接种到新生的小鼠体内时， 迅速扩散并引发多种肿瘤。其途径和机制 病毒感染细胞的方式将在培养中使用电子技术进行研究 显微镜和去卷积荧光显微镜以及各种 免疫学和生化方法。具体的目标是针对 确定内部化的模式，以及进入 原子核。结构蛋白有缺陷的突变病毒影响 参赛作品将被特色化。 我们已经鉴定出一种近交系小鼠，它对多瘤具有高度的抵抗力。 感染。这种寄主抗性的遗传和生理基础将是 通过与易感小鼠杂交和使用细胞培养进行研究 来自耐药小鼠。这项调查的最终目标是 是对东道主行为模式的识别和理解 阻止病毒传播的抗性基因。我们还将尝试分离出 野家鼠的多瘤病毒，并确认是否存在这些病毒 实验室菌株中鉴定的致病因子 病毒的属性。

The overall goal of this project is a better understanding of the mechanisms of cell entry and spread by polyoma virus. This virus infects and transforms rodent cells in culture. When inoculated into newborn mice, it spreads rapidly and induces a wide array of tumors. The pathway and mechanisms by which the virus infects cells will be studied in culture using electron microscopy and deconvolution fluorescence microscopy along with various immunological and biochemical methods. Specific aims are directed to identifying the mode of internalization, and the pathway of entry into the nucleus. Mutant viruses with defects in the structural proteins that affect entry will be characterized. We have identified an inbred mouse strain which is highly resistant to polyoma infection. The genetic and physiological bases for this host resistance will be investigated in crosses with susceptible mice, and by use of cell cultures derived from the resistant mice. The final goal of this line of investigation is the identification and understanding of the mode of action of the host resistance gene which blocks virus spread. We will also attempt to isolate polyoma virus from wild house mice and confirm the presence or absence of those determinants identified in laboratory strains which confer pathogenic properties to the virus.