CORE--BLOOD AND TISSUE SPECIMEN

描述(由申请人提供)：该中心将作为血液和组织研究的重要资源中心，这些研究是完成与脑出血和缺血性梗塞的侵略性介入治疗相关的研究项目所不可或缺的。它将为研究患者的血液和组织分析带来与中风发病机制相关的形态解剖学技术方面的丰富专业知识，并为主要研究人员提供从研究患者的活检和尸检样本中提取的脑组织样本的途径。该核心将支持项目1，即磁共振抢救试验，在该试验中，血管内血栓材料将在治疗性血栓切除过程中被移除。该核心将帮助研究人员研究血栓形成的生化机制，以及它在脑梗塞演变中的可能重要性，并将帮助研究人员将他们的发现与血栓的光学显微镜/超微结构特征相关联。核心也将 为项目2提供支持，即血红素手术试验，在该试验中，脑组织起源于血块 对自发性脑(实质)出血患者进行的疏散将仔细评估出血的可能原因和对脑内血液的组织反应，并为利用定性、实时RT-PCR和基因芯片研究差异基因表达做好准备。核心中心将存储适当患者的血液样本用于DNA分析，并在适当审查他们的研究目标和项目后将这些样本提供给研究人员。该中心将作为加州大学洛杉矶分校SPOTRIAS研究人员的重要“血液和脑组织”资源，以及对急性缺血性和出血性中风的治疗感兴趣的更大的研究人员网络。

DESCRIPTION (provided by the applicant): This Core will serve as a vital resource center for blood and tissue-based studies integral to the completion of investigational projects, related to aggressive, interventional treatment of cerebral hemorrhage and ischemic infarcts. It will bring to analyses of blood and tissues derived from study patients a wealth of expertise in morphoanatomical techniques pertinent to stroke pathogenesis, and provide access to key investigators of samples of brain tissue derived from biopsy and autopsy specimens of study patients. The core will support Project 1, the MR RESCUE trial, in which intravascular thrombus material will be removed in the course of therapeutic thrombectomy. The core will assist investigators studying the biochemical pathogenesis of thrombus formation, and its possible importance in evolution of cerebral infarcts, and will assist investigators in correlating their findings with light microscopic/ultrastructural features of the thrombi. The core will also provide support to Project 2, the HEME Surgery trial, in which brain tissue originating from 'clot' evacuations carried out in patients with spontaneous intracerebral (parenchymal) hemorrhage will be carefully evaluated for both the likely etiology of hemorrhage and the tissue response to intracerebral blood, and readied for studies of differential gene expression employing qualitative, real-time RT-PCR and cDNA arrays. The Core will store blood samples from appropriate patients for DNA analysis, and provide these to researchers following appropriate review of their investigational goals and projects. The Core will serve as an important 'blood and brain tissue' resource to SPOTRIAS investigators at UCLA, and a larger network of investigators with interests in the treatment of acute ischemic and hemorrhagic stroke.