Upgrating Biomedical Facilities--UPR-Rio Piedras Campus

描述(由申请人提供)：波多黎各大学(UPR)-里约皮德拉斯校区自然科学学院的SCORE方案目前有17名教员研究员、5名研究助理和13名实验室技术人员参与生物和化学系的17个研究项目。最近，在基因组学和功能基因组学方面继续发展具有竞争力的研究核心被确定为生物系的关键优先事项。这包括今后在这一领域招聘两名新的研究人员和扩大测序设施。这项申请要求提供资金，用于升级测序和基因分型设施，使其能力翻倍，建立连接核心基因组设施的计算基础设施，并实施实验室信息管理系统。在化学系，核磁共振实验室继续为研究界服务，提供先进的核磁共振分析服务、培训和咨询，使岛上的科学家能够调查复杂的系统，并获得关于其结构和组成的明确细节。尽管如此，有必要用配备附件的更强大的仪器来取代已有15年历史的过时仪器，以实现高分辨率的核磁共振波谱，并在传统的非梯度脉冲序列所需时间的一小部分内获得更高质量的数据。为了实现核磁共振设施的升级，该应用程序正在为一个新的500 MHz多核脉冲磁场梯度系统申请资金。 基因组研究核心设施的扩展、升级和整合将显著提高生成和管理序列、基因和基因表达数据的能力，这些数据是现代生物医学研究的基石。这将影响来自生物和化学系的11名教职调查人员。以类似的方式，新的核磁共振波谱仪将允许7SCORE研究人员探索新的研究问题，进一步推进他们的研究目标，并通过新一代选择性ID实验、普通2D实验的梯度版本和更新的高级混合2D核磁共振序列在更短的时间内获得更高质量的数据。

DESCRIPTION (provided by applicant): The SCORE Program at the University of Puerto Rico (UPR)-Rio Piedras Campus, College of Natural Sciences currently involves 17 faculty investigators, 5 research associates and 13 laboratory technicians in 17 research projects at the Departments of Biology and Chemistry. Recently, the continued development of a competitive research core in genomics and functional genomics was identified as a key priority for the Biology Department. This included the future recruitment of two new researchers in this area and the expansion of the sequencing facility. This application requests funds for the upgrading of the sequencing and genotyping facilities by doubling its capacity, to establish computational infrastructure to link the core genomic facilities and to implement a Laboratory Information Management system. In the Department of Chemistry, the Nuclear Magnetic Resonance (NMR) laboratory continues to serve the research community by providing advanced NMR analytical services, training and consultation that enables scientists around the island to investigate complex systems and obtain unambiguous details about their structure and composition. Nonetheless it has become necessary to replace a fifteen year old obsolete instrument with a more powerful instrument equipped with accessories that will allow for high resolution NMR spectroscopy and for the acquisition of higher quality data in a fraction of the time taken by traditional non-gradient pulse sequences. To effect the upgrade of the NMR facilities this application is requesting funds for a new 500 MHz multinuclear, pulsed field gradient system. The expansion, upgrade and integration of the core facilities for genomic research will significantly improve the ability to generate and manage sequence, genotype and gene expression data, a cornerstone of modern biomedical research. This will impact 11 faculty investigators from the Biology and Chemistry Departments. In a similar manner the new NMR spectrometer will allow 7 SCORE investigators to explore new research problems, further advance their research goals and obtain higher quality data in shorter times with the new generation of selective ID experiments, the gradient versions of the common 2D experiments and newer advanced hybrid 2D NMR sequences.