Enhanced Cell Separation Technology for COBRE P20RR15587

描述（由申请人提供）： 最初的COBRE拨款（P20 RR 15587）于2000年9月授予爱达荷州大学（U of I）研究人员，并资助“宿主-病原体相互作用的分子和细胞基础研究中心”。该中心的总体目标是建立在我们目前在微生物发病机制的核心力量，并建立我的U作为一个首屈一指的机构，在这个主题重点领域的国家认可的生物医学计划。到目前为止，COBRE计划已经成功地使我们能够将几位已建立的生物医学科学家的计划与新聘请的终身教职人员的计划相结合。自从我们最初的COBRE提案提交以来，每个COBRE调查员所需的可用技术都有了发展。COBRE补充申请是根据每个项目正在进行的研究制定的，同时考虑到我们小组和I大学和爱达荷州其他地方的其他生物医学研究人员未来的赠款申请。具体目标1将通过提供最先进的细胞分离技术来提高COBRE中心合作研究者的生物医学研究竞争力和生产力。我们建议通过购买两种关键工具来实现这一目标：1）PALM机器人-微束激光消融和细胞回收系统;以及2.）BD Biosciences免疫细胞计数系统FACSAria流式细胞仪。PALM机器人微束激光消融和细胞检索系统将提供目前在I大学或爱达荷州任何机构都无法获得的先进研究能力。该系统的优点是能够进行非接触式激光显微切割。该系统是我们许多细胞生物学项目的关键，这些项目涉及细胞或细胞器的分离和检索，然后是DNA，RNA或其他类型的分子测定。U of I流式细胞仪设施目前配备了仅具有基本细胞分选能力的FACSCalibur。FACSAria流式细胞仪将大大增强我们的分选能力;这是每个COBRE研究者的关键需求。此外，FACSAria系统的模块化设计允许安装其他专门单元，以满足未来的研究需要。 本提案中要求的两种仪器和配件将直接受益于（i）我们的COBRE中心的核心研究人员，用于调查宿主病原体相互作用的分子和细胞基础，（ii）最近聘请的终身教职教师，由爱达荷州的第二个资助的COBRE（进化过程中心）提供支持，以及（iii）由爱达荷州BRIN项目促进的其他生物医学研究工作。

DESCRIPTION (provided by applicant): The initial COBRE grant (P20RR15587) was awarded to University of Idaho (U of I) investigators in September 2000 and funded the "Center for Investigating the Molecular and Cellular Basis of Host-Pathogen Interactions". The overall objective of this center is to build on our current core of strength in microbial pathogenesis and establish the U of I as a premier institution with a nationally recognized biomedical program in an area with this thematic focus. Thus far, the COBRE program has been successful in enabling us to integrate the programs of several established biomedical scientists with those of newly hired, tenure-track, faculty. Since submission of our original COBRE proposal, there has been an evolution in the technology available and required by each of the COBRE investigators. This COBRE supplemental request was developed based on research ongoing in each of the projects, while taking into consideration future grant requests by our group and by other biomedical researchers at the U of I and elsewhere in Idaho. Specific Aim 1 will enhance the biomedical research competitiveness and productivity of COBRE Center coinvestigators by providing state-of-the-art cell separation technology. We propose to accomplish this aim by purchasing two critical instruments: 1.) PALM Robot-Microbeam Laser Ablation and Cell Retrieval System; and 2.) BD Biosciences Immunocytometry Systems FACSAria Flow Cytometer. The PALM Robot-Microbeam Laser Ablation and Cell Retrieval System will provide advanced research capabilities that are currently not available at the U of I or at any institution in Idaho. The advantage of this system is its ability to perform non-contact laser microdissection. This system is key to many of our cell biology projects that involve cell or organelle isolation and retrieval followed by DNA, RNA, or other types of molecular assays. The U of I Flow Cytometry Facility is currently equipped with a FACSCalibur that possesses only rudimentary cell-sorting capability. The FACSAria Flow Cytometer will substantially enhance our sorting capability; a critical need for each of the COBRE investigators. Furthermore, the modular design of the FACSAria system allows for installation of other specialized units in response to future research needs. The two instruments and accessories requested in this proposal will directly benefit (i) the core researchers in our COBRE Center for Investigating the Molecular and Cellular Basis of Host Pathogen Interactions, (ii) recently hired tenure track faculty being supported by Idaho's second funded COBRE (Center for Processes in Evolution) and (iii) other biomedical research endeavors facilitated by the Idaho BRIN project.