Neural Development and Genetics of Zebrafish

描述（由申请人提供）：本提案要求部分支持 为期两周的密集课程，为高级研究生，博士后 研究员和独立调查员，重点是发展和 斑马鱼的遗传学，特别强调神经系统。第一 一周的课程涵盖了斑马鱼的早期发育和遗传学， 相关的方法论，而第二周的重点是神经 系统上午和下午一般用于背景讲座 实验室练习和晚上的研究讲座。每一天都在 由一名或两名初级教员协助的高级教员的监督 教职员工。本课程仅限16名学生。第一次演讲 周涵盖斑马鱼的发育阶段，化学和插入 诱变、基因定位、早期发育和斑马鱼饲养。在 第二周，向学生介绍斑马鱼的神经系统， 它的发展，以及神经系统突变体的分析。具体领域 第二周包括前脑和后脑，脊髓， 眼睛和视网膜，顶盖，以及斑马鱼行为的各个方面。实验室练习 在第一周向学生介绍野生型和突变型胚胎的发育， 基因定位、核酸注射、细胞移植等技术， 原位杂交和单细胞标记。在第二周， 学生学习斑马鱼神经系统的发育。他们学习 轴突寻路技术，如何回填神经元，移植眼睛， 对野生型和突变型动物进行行为测试。

DESCRIPTION (provided by applicant): This proposal requests partial support for an intensive two-week course for advanced graduate students, postdoctoral fellows and independent investigators that focuses on the development and genetics of zebrafish with special emphasis on the nervous system. The first week of the course covers the early development and genetics of zebrafish, and the relevant methodologies, whereas the second week focuses on the nervous system. Mornings and afternoons are generally devoted to background lectures and laboratory exercises and evenings to research lectures. Each day is under the supervision of a senior faculty member who is assisted by one or two junior faculty members. The course is limited to 16 students. Lectures in the first week cover the developmental stages of zebrafish, chemical and insertional mutagenesis, gene mapping, early development, and zebrafish husbandry. In the second week, the students are introduced to the nervous system of zebrafish, its development, and the analysis of nervous system mutants. Specific areas covered in the second week include the forebrain and hindbrain, spinal cord, eye and retina, tectum, and aspects of zebrafish behavior. Laboratory exercises in the first week introduce students to developing wildtype and mutant embryos, techniques of gene mapping, nucleic acid injections, cell transplantation, in situ hybridization, and the labeling of single cells. In the second week, students study the development of the zebrafish nervous system. They learn axonal pathfinding techniques, how to back-fill neurons, transplant eyes, and perform behavioral tests on wildtype and mutant animals.