Core-- Administration

建立了体外模型和细胞培养核心，作为所有项目的资源 PPG。具体目标是： 1.获取正常组织和CF组织。 2.人呼吸道上皮细胞体外模型的建立。 3.体外模型和天然上皮细胞的特性。 A.电生理分析。 B.形态评价。 C.CF细胞的基因分型。 4.研究和开发基因转移、呼吸道上皮、细胞生物学和粘膜下腺研究的新方法和新模型。 5.为PPG的研究人员生产培养细胞。 6.呼吸道上皮细胞冷冻库的建立。 7.在爱荷华大学和其他机构向研究人员传授 开发体外模型系统。 8.与组织采集和细胞培养相关的记录保存。 9.细胞和动物获得监管委员会的批准和记录保存 学习。 10.向其他机构的调查人员提供细胞、质粒和病毒。 这一核心将高度上进和技术成熟的员工的专业知识带到 PPG调查员的好处。因此，它允许PPG调查人员专注于他们的 在与CF基因转移高度相关的问题上具有独特的专业知识。

The In Vitro Models and Cell Culture Core is established as a resource for all projects in the PPG. The specific aims are: 1. Acquisition of normal and CF tissue. 2. Generation of in vitro models of human airway epithelia. 3. Characterization of in vitro models and native epithelia. a. Electrophysiologic analysis. b. Morphologic evaluation. c. Genotyping of CF cells. 4. Research and development of new methods and models for the study of gene transfer, airway epithelial, cell biology, and submucosal glands. 5. Production of cultured cells for investigators in the PPG. 6. Development of a cryogenic bank of airway epithelial cells. 7. Teaching investigators at the University of Iowa and other institutions the methods for developing in vitro model systems. 8. Record keeping relevant to tissue acquisition and cell culture. 9. Obtaining approval from regulatory committees and record keeping for cell and animal studies. 10. Provision of cells, plasmids, and viruses to investigators at other institutions. This Core brings the expertise of a highly motivated and technically sophisticated staff to the benefit of the PPG investigators. As a result, it allows PPG investigators to focus their unique expertise on questions of high relevance to CF gene transfer.