Core A- Cell Purification and Analysis Laboratory Core

建议为该中心建立一个共享的细胞采购和分析实验室， 程序项目。 该实验室将在所有领域发挥重要作用 项目的四个子项目 这个核心将协调和 进行流式细胞术和免疫组织化学用于纯化和/或 用于拟议研究的造血细胞分析。 本 流式细胞术研究实验室将成为拟议的一部分 核心单元完全配备了最先进的仪器， 所需的复杂的流式细胞术程序的性能 建议的研究。 流式细胞术实验室目前位于 一个单独的400平方英尺的房间在五楼的医疗 研究和图书馆大楼，并将搬迁到一个更大的 新研究大楼一楼的实验室空间 预计在1996年完成。 实验室目前 由两名合格的全职员工提供支持 接受过流式细胞术广泛培训的操作员。 一个 预计将有更多员工加入核心部门， 负责组织和分选细胞的采购和制备 参与拟议项目的研究人员的人口。 共享细胞采购和分析实验室将提供 参与者在该计划项目的设备的经济使用， 空间以及进行所需的必要细胞群 研究 免疫组织学实验室将成为拟议核心的一部分 该设备配备齐全，可对动物和人类进行免疫鉴定 造血和非造血细胞， 细胞衍生、细胞活化或癌基因产物。 此外 实验室可以使用 单克隆抗体，并可通过过氧化物酶DNA评估细胞凋亡 和标记技术。 实验室位于一个210平方英尺的 血液病理学部内的设施 病理科在四楼重症监护室医院 实验室是 目前由两名全职技术人员组成，他们拥有广泛的 具有研究和常规免疫组织学经验。

A shared cell procurement and analysis laboratory is proposed for the program project. This laboratory will serve an essential function in all four subprojects of the program project. This core will coordinate and perform flow cytometry and immunohistochemistry for purification and/or analysis of hematopoietic cells for the proposed studies. The present flow cytometry research laboratory which will be part of the proposed core unit is fully equipped with state of the art instrumentation for the performance of sophisticated flow cytometry procedures required by the proposed studies. The flow cytometry laboratory is currently located in a separate 400 square feet room on the fifth floor of the Medical Research and Library Building and will be relocated into a larger laboratory space on the first floor of the new Research Building projected to be completed in 1996. The laboratory activity is currently supported by two full time employees both of whom are well qualified operators who have received extensive training in flow cytometry. An additional employee is expected to join the core unit with the sole responsibility of procuring and preparing tissues and sorted cell populations for researchers involved in the proposed program project. The shared cell procurement and analysis laboratory will provide for participants in the program project an economical use of equipment and space as well as necessary cell populations required for the conduct of the research. The immunohistology laboratory which will be part of the proposed core unit is fully equipped to immunocharacterize animal and human hematopoietic and non-hematopoietic cells using antibodies specific for cell derivation, cell activation or oncogene products. In addition the laboratory can perform cytokinetic analysis of the specimens using the monoclonal antibodies and can assess cell apoptosis by a peroxidase DNA and labeling technique. The laboratory is located in a 210 square feet facility within the Division of Hematopathology of the Department of Pathology at the fourth floor of the I.U. Hospital. The laboratory is currently staffed by two full time techologists who have extensive experience in both research and routine immunohistology.