Novel plant-based Antimicrobials against oral pathogens

描述：一种新的生物技术平台，“天然产物基因组学”已经开发出来，用于寻找基于植物的药物，它利用高通量筛选来识别大量突变植物细胞培养物中的活性化合物。这项技术很可能涉及到植物能够产生的所有代谢产物。在这方面，植物，特别是它们的根，经常受到细菌和真菌病原体的攻击，这些病原体与感染人类口腔的病原体有相似之处。因此，植物的防御机制是抗菌化合物的潜在来源，可能对口腔健康有价值。这项申请是为了开发方法，将允许新的平台技术应用于识别和分离对口腔病原体有活性的植物化合物。为此，首先有必要开发一种可用于非常小的植物细胞培养物的超高通量抗菌活性筛选。该屏幕的开发代表了该STTR第一阶段的可交付成果。这一发展有两个主要方面。首先，必须开发合适的病原体培养物，其中可以使用细胞损伤/杀伤指标来评估植物培养分泌物的功效。其次，必须开发一种将病原体培养物暴露于突变植物细胞培养物的机制。该应用是为了探索病原体和植物培养物可以在“匹配”的96孔板上作为共培养系统生长的可能性，植物培养物可以在带有半透膜的孔中生长。然后将培养皿接触24小时，培养基仅由半透膜隔开，使培养物的分泌产物扩散到病原体上。如果成功，该STTR将代表天然产物基因组学技术在口腔健康药物领域的极有价值的转移。通过这种方法发现的化合物应该具有相当大的经济和医学价值，作为控制口腔病原体的专有药物。

DESCRIPTION: A novel platform biotechnology, "natural products genomics" for seeking plant-based medicines has been developed which utilizes high throughput screening to identify active compounds in large populations of mutant plant cell cultures. This technology likely accesses the entire range of metabolic products of which the plant is capable. In this regard, plants, particularly their roots, are constantly under attack from bacterial and fungal pathogens that share similarities with those that infect the human oral cavity. The defense mechanisms of plants are therefore a potential source of antimicrobial compounds that may be of value in oral health. This application is to develop methods that will allow the novel platform technology to be applied to the identification and separation of plant compounds that are active against oral pathogens. For this, it is first necessary to develop an ultra high-throughput screen for antimicrobial activity that can be used with very small plant cell cultures. The development of this screen represents the deliverables for Phase I of this STTR. There are two major aspects to this development. First, appropriate pathogen cultures must be developed in which an indicator of cell damage/killing can be used to evaluate efficacy of plant culture secretions. Second, a mechanism for exposing the pathogen cultures to the mutant plant cell cultures must be developed. The application is to explore the possibility that pathogen and plant cultures can be grown on "matching" 96 well plates as co-culture systems, with plant cultures grown in wells with a semi-permeable membrane. The plates can then be placed in contact for 24h, with culture media separated only by the semi-permeable membrane, allowing secreted products from the cultures to diffuse onto the pathogens. If successful, this STTR will represent an extremely valuable transfer of the natural products genomics technology into the field of oral health medications. Compounds discovered by this method should be of considerable economic and medical value as proprietary medicines for the control of oral pathogens.