New Brunswick 100 Liter Fermentation Facility in Memphis

描述（由研究者提供）：我们申请资金在田纳西州孟菲斯的圣裘德儿童研究医院（圣裘德）建立一个大规模（高达100升）细菌培养设施。该设施将为孟菲斯的广大科学家社区提供服务，包括田纳西大学健康科学中心（U。田纳西州），圣裘德的研究生教育合作伙伴，和孟菲斯大学（美国）。孟菲斯），许多合作研究项目的合作伙伴。该设施的目的是能够大规模生产蛋白质和其他生物分子，用于结构，生物化学和药物发现研究。 拟议的发酵设施将影响上述三个机构的各种NIH资助的生物医学研究项目，从基本生物功能的结构研究和基于结构的药物设计，到生产小分子作为合成新疗法和高通量小分子筛选的中间体。总的来说，发酵设施将有助于推进由分布在三个机构之间的十几个R01，P01，U01或R37应用程序资助的研究。对正在进行的研究的主要贡献将是能够大规模生产生物分子样品，特别是蛋白质。在某些情况下，大规模的蛋白质生产已经在进行中，但以时间效率极低的方式进行。进入拟议的发酵罐将使科学家能够将更多的时间集中在生物化学和结构研究上，而不是蛋白质生产上。在其他情况下，由于表达水平低，某些蛋白质的生产目前无法实现。这一类别的研究将通过进入100升发酵罐而成为可能。设立这一基金的另一个好处是促进机构内和机构间的合作。虽然这里的许多参与者已经合作，但使用共享发酵设施将促进团体之间的互动，特别是在实际执行发酵实验的研究生和博士后学员的层面。

DESCRIPTION (provided by investigator): We request funds to establish a large-scale (up to 100 liters) bacterial culture facility in Memphis, TN, to be located at St. Jude Children's Research Hospital (St. Jude). This facility will be available to serve a broad community of scientists in Memphis, including those at St. Jude, The University of Tennessee Health Sciences Center (U. Tennessee), St. Jude's partner in graduate education, and The University of Memphis (U. Memphis), a partner in many collaborative research projects. The purpose of this facility is to enable the large-scale production of proteins and other biomolecules for structural, biochemical and drug discovery studies. The proposed Fermentation Facility will impact a wide variety of NIH-funded biomedical research projects at the three institutions noted above, ranging from structural investigations into basic biological function and structure-based drug design, to the production of small molecules as intermediates for the synthesis of new therapeutics and high-throughput small-molecule screening. Overall, the Fermentation Facility will contribute to advancing research funded by more than one dozen R01, P01, U01, or R37 applications distributed between the three institutions. The principal contribution to ongoing research will be to enable large-scale production of biomolecule samples, especially proteins. In some cases, large-scale protein production is already under way but is performed in a highly time inefficient manner. Access to the proposed fermentor will allow scientists to focus much more of their time on biochemical and structural studies rather than on protein production. In other cases, production of certain proteins is currently out of reach due to low expression levels. Studies in this category will be made possible through access to the 100-liter fermentor. An additional benefit associated with creating this facility is the promotion of intra- and inter-institutional collaboration. While many of the participants here already collaborate, use of a shared Fermentation Facility will foster interactions between groups, especially at the level of graduate students and postdoctoral trainees who will actually perform fermentation experiments.