Triple Resonance Cryoprobe System for a 600 MHz NMR

请为布兰代斯大学现有的现代瓦里安Inova 600 MHz核磁共振仪提供资金，用于低温冷却、超高灵敏度三重共振、1H{13C/15N}、5 mm探头和相关的闭合循环低温系统。低温探头技术已被证明为研究具有生物重要性的大分子提供了显著的信噪比改进。600 MHz光谱仪目前主要用于高分辨率、高灵敏度的多维核磁共振实验，通常是在溶液中使用同位素标记的(15N、13C、2H)生物样品。低温探头/低温平台将显著提高仪器的灵敏度和吞吐量。布兰代斯大学的三名研究人员以及塔夫茨大学和波士顿大学的两名科学家是低温探测器的最初主要用户。通过低温探测器获得的灵敏度的提高将使研究小组能够进行目前还不能进行的实验 在现有光谱仪上具有实用价值。技术要求很高的项目，如研究 (I)催化过程中的酶，(Ii)短寿命的大分子物种和(Iii)大的蛋白质和只能获得低浓度样品的蛋白质/蛋白质复合体的动力学和结构将从信噪比的提高中获得极大的好处。最后，由于核磁共振时间目前是一个限制因素，系统吞吐量的增加将加速NIH支持的研究项目。位于布兰代斯的核磁共振设施拥有维护和操作该仪器的技术技能。

Funding is requested for a cryogenically-cooled, ultra high-sensitivity triple resonance, 1H{13C/15N}, 5 mm probe and associated closed-cycle cryogenic system for an existing modern Varian Inova 600 MHz NMR spectrometer at Brandeis University. The cryoprobe technology has been demonstrated to provide a significant improvement in signal-to noise ratios for studies of biologically important macromolecules. The 600 MHz spectrometer is currently heavily used for high-resolution, high-sensitivity multidimensional NMR experiments, typically with isotopically labeled (15N, 13C, 2H) biological samples in solution. The cryoprobe/cryoplatform will significantly increase the sensitivity and throughput of the instrument. Three investigators from Brandeis University along with two scientists from Tufts University and Boston University constitute the initial major users of the cryoprobe. The increase in sensitivity gained by the cryoprobe will allow the research groups to perform experiments that are currently not practical on the existing spectrometer. Technically demanding projects such as studies of the dynamics and structure of (i) enzymes during catalysis, (ii) shortly-lived macromolecular species and (iii) large proteins and protein/protein complexes for which only low concentrated samples can be reached will greatly benefit from the increase in signal-to noise ratio. Finally, the increased throughput of the system will accelerate the NIH supported research projects since NMR time is currently one limiting factor. The technical skills are available to maintain and operate the instrument at the NMR facility at Brandeis.