TRANSCRIPTION OF ACANTHAMOEBA RNA PLYMERASE II GENES

描述(摘自申请者摘要)：卡氏棘阿米巴 是一种小的、自由生活的阿米巴，是棘阿米巴的病原体 角膜炎。棘阿米巴可以支持几种人的细胞内生长 病原体，并可能是细菌的重要环境储存库， 这需要细胞内的生长。棘阿米巴的特征是有丝分裂 生长周期，以及在不利条件下形成包囊 都会遇到。分化的分子机制主要是 未知。这是一项研究基因表达机制的建议。 生长和分化。对生长至关重要的两个基因，TBP基因和 调节它的因子TPBF的基因将在 体外和体内。编码转录调控的包囊的基因 特定蛋白(CSP21)已经被克隆，这为 描述细胞特异性基因表达的机制。

DESCRIPTION (Adapted from the Applicant's Abstract): Acanthamoeba castellanii is a small, free living amoeba that is the causative agent for Acanthamoeba keratitis. Acanthamoeba can support intracellular growth for several human pathogens and may represent a significant environmental reservoir for bacteria, which require intracellular growth. Acanthamoeba is characterized by a mitotic growth cycle, and formation of an encapsulated cyst when unfavorable conditions are encountered. The molecular mechanisms of differentiation are largely unknown. This is proposal to characterize mechanisms of gene expression during growth and differentiation. Two genes essential for growth, the TBP gene and the gene of the factor, which regulates it, TPBF, will be characterized in vitro and in vivo. The gene encoding a transcriptionlly regulated, cyst specific protein (CSP21) has been cloned, providing an opportunity to characterize the mechanisms of cell-specific gene expression.