Obesity-mediated dysregulation in the expression & action of myostatin:

全文标题：肥胖导致的肌肉抑制素的表达和作用失调：一种导致年龄相关性石棺减少和胰岛素敏感性的新的内在机制。该项目涉及广泛的生化、分子和细胞生物学技术(包括原代人类细胞培养、转录分析和生物信息学)在从不同年龄和体重类别的健康志愿者获得的脂肪和骨骼肌组织中的应用。这一方法将帮助我们理解肥胖和脂肪在人体骨骼肌中积累影响肌肉抑制素(一种已知的骨骼肌质量负调节因子)调节的细胞机制(S)，以及这如何导致骨骼肌减少(肌肉质量随年龄减少)和胰岛素抵抗(代谢综合征的特征之一)的发展，并确定候选的治疗策略。这项工作产生的知识将对改善肌肉骨骼老化的干预措施的发展产生重要影响，肌肉骨骼老化是21世纪最大的健康挑战之一。博士生将受益于在英国两个备受尊敬的独立实验室工作，获得一系列人体体内和体外研究技能。这名学生预计将在世界上最大的营养、健康和福利公司雀巢的研究实验室中花费相当长的时间(6-9个月)，接受转录分析和生物信息学方面的培训。

Full title: Obesity-mediated dysregulation in the expression and action of myostatin: A novel intrinsic mechanism that contributes to age-related sarcopenia and sensitivity to insulinThe project involves the application of a wide range of biochemical, molecular and cellular biology techniques (including primary human cell culture, transcriptomic analysis and bioinformatics) in adipose and skeletal muscle tissues obtained from healthy volunteers from different age and weight categories. This approach will help us understand the cellular mechanism(s) by which adiposity and fat accumulation within human skeletal muscle affect the regulation of the protein myostatin (a known negative regulator of skeletal muscle mass), how this contributes to sarcopenia (loss of muscle mass with age) and the development of insulin resistance (one of the hallmarks of the metabolic syndrome), and to identify candidate therapeutic strategies. The knowledge generated from this work will have important implications for the development of interventions to improve musculoskeletal ageing, one of the biggest health challenges of the 21st century.The PhD student will benefit from working in two highly regarded, independent laboratories in UK acquiring a range of human in vivo and ex vivo research skills. The student is expected to spend considerable time (6-9 months) in the research laboratories of Nestle, the world's largest Nutrition, Health and Welfare company, receiving training in transcriptomic analysis and bioinformatics.