Expanding Combinatorial Space in Bacterial Libraries for Synthetic Biology Applications

虽然合成和系统生物学渴望生物系统的设计，但组合方法仍然是快速分离功能设计的有效策略。因此，高质量的文库是必不可少的，它们的组装取决于多个实际和技术问题：（1）合成的鲁棒性，（2）稳定性和（3）序列空间的采样。最近开发了一种新的方法来建立大型多组分体内文库，我们希望通过改进（1）和开发新的检测方法来证实（3），将该方法扩展到研究合成药物和生物过程微区室封装的适应性。

Although Synthetic and Systems Biology aspire to the design of biological systems,combinatorial approaches remain efficient strategies to rapidly isolate functional designsfrom a vast number of possible designs. High-quality libraries are therefore essential andtheir assembly hinges on multiple practical and technical issues: (1) robustness of synthesis,(2) stability and (3) sampling of the sequence space. Having recently developed a novelapproach to establish large multi-component in vivo libraries, we want to expand theapproach towards studying the fitness of synthetic phages and microcompartmentencapsulation of biological processes - by improving (1) and developing novel assays toconfirm (3).