High throughput selection and character. of aptamers

高通量，自动化选择方法将采用艾灵顿实验室在 得克萨斯大学奥斯汀分校，以产生针对HIV-1 Rev，达特，逆转录病毒 转录酶、RNase H和RRE。适体将由公司进行表征 Accacia，随后在病毒感染的细胞培养模型中通过Prasad 以及约翰逊的动物模型 在哈佛的实验室。选定的适体还将用于生成用于映射的芯片 表位和用于检测抗性突变体。病毒耐药性的发展 将通过对已经获得的蛋白进行重新选择来进一步解决 耐药突变最终，这些选择实验应该加强 开发高效的艾滋病基因疗法，也将提供独特的 深入了解病毒耐药性的进化。

High-throughput, automated selection methods will be employed by the Ellington lab at the University of Texas at Austin to generate aptamers against HIV-1 Rev, Tat, reverse transcriptase, Rnase H, and the RRE. Aptamers will be characterized by the company Accacia, and subsequently assayed in cell culture models of viral infection by the Prasad lab at the Albert Einstein College of Medicine, as well as in animal models by the Johnson lab at Harvard. The selected aptamers will also be used to generate chips for mapping epitopes and for the detection of resistance mutants. The development of viral resistance will be further addressed by conducting re-selections against proteins that have acquired resistance mutations. Ultimately, these selection experiments should potentiate the development of highly efficacious gene therapies for AIDS and will also provide unique insights into the evolution of viral resistance.