Core--HUMAN TISSUE RESOURCES

人体组织核心设施是在一名基础科学家和一名病理学家的领导下建立的，以确保前列腺组织的采购和利用得到优化。材料将在手术切除后立即获得，并将被分割成新的材料用于器官和细胞培养。更多的正常和恶性部分将被快速冷冻，以供研究项目使用。Core还将为研究项目提供石蜡包埋和冷冻材料的激光捕获显微解剖(LCM)。所有相关信息将被传输到核心D以进行数据库录入。核心B的组件： 1.组织获取2.器官培养3.建立原代前列腺细胞系 4.正常腺体和肿瘤腺的激光捕获显微解剖(LCM)。

The Human Tissue Core Facility has been established under the leadership of a basic scientist and a pathologist to ensure that prostate tissue procurement and ultilization of the tissue is optimized. Material will be obtained immediately after surgical removal and will be partitioned into fresh material for organ and cell culture. Additional normal and malignant portions will be snap frozen for use by the research projects. The Core will also provide laser capture microdissection (LCM) of both paraffin-embedded and frozen material for the research projects. All relative information will be transmitted to Core D for database entry. Components of Core B: 1. Tissue procurement 2. Establish organ cultures 3. Establish primary prostate cell lines 4. Laser capture microdissection (LCM) of normal and neoplastic glands.