Development of a DNA amplification-free DNA detection assay.

DNA扩增技术对于从癌症到病毒的广泛生物系统的研究、检测和表征至关重要。聚合酶链反应（PCR）是最常用的技术，但近年来也发现了等温扩增方法的使用。这些方法依赖于引物组和酶来扩增靶DNA序列，然后通常使用荧光DNA结合染料进行读出。虽然这些技术是强大的，但它们在一系列环境中也有缺点，要么是由于需要专门的设备，要么是灵敏度过高，没有特异性amplification.In这里，我们建议开发一种新的平台技术，允许研究和检测DNA（或RNA），而不需要专门的设备或DNA扩增。在这里，乙酰氧基甲基醚修饰的染料将与催化实体结合，以在目标遗传物质存在下产生荧光信号。

DNA amplification techniques are essential for the study, detection and characterisation of a wide range of biological systems from Cancer to viruses. Polymerase chain reaction (PCR) is the most commonly used techniques but isothermal amplification approaches have also found use in recent years. These approaches rely on primer sets and enzymes to amplify target DNA sequences, followed by readout, typically using a fluorescent DNA binding dye. While these techniques are powerful they also have drawbacks in a range of environments either due to the need for specialised equipment or over sensitivity and none specific amplification.Here we propose to develop a novel, platform technology that allows for the study and detection of DNA (or RNA) without the need for specialised equipment or DNA amplification. Here acetoxymethyl ether modified dyes will be combined with catalytic entities to yield fluorescent signals in the presence of target genetic material.