Point Detection of Pathogens in Oral Samples via UPT

描述(由申请人提供)：来自宾夕法尼亚大学牙科医学院和工程与应用科学学院的多学科科学家小组与唾液诊断领域的主要公司OraSure Technologies的研究人员一起生成了这份申请。我们建议开发一种新的、基于口腔的系统，旨在用于多种传染病的诊断。该技术平台将允许同时分析病毒和/或细菌抗原和核酸，以及针对这些病原体的抗体。该项目的重点是艾滋病毒和艾滋病毒抗体，但适用于任何传染病的检测，包括与生物恐怖主义制剂有关的疾病。 该提案分为三个研究领域。(1)样品采集，(2)口腔样品的微流控处理，以及(3)利用上转换荧光粉技术(UPT)的分析检测。基本的UPT系统已经存在，并已用于对滥用药物、病原体抗体和核酸进行定量。我们现在提出一项计划，将这些独立的分析结合到一个单一的系统中，该系统可以在现场使用，分析多达200个目标分析物。

DESCRIPTION (provided by applicant): A multidisciplinary group of scientists from the University of Pennsylvania School of Dental Medicine and the School for Engineering and Applied Sciences have joined with investigators from OraSure Technologies, the dominant company in salivary diagnostics, to generate this application. We propose to develop a novel, oral-based system, designed for use in the diagnoses of multiple infectious diseases The technology platform will permit the simultaneous analyses of viral and/or bacterial antigens and nucleic acids, as well as antibodies to these pathogens. The project focuses on HIV and antibodies to HIV, but is applicable to detection of any infectious disease including those associated with agents of bioterrorism. The proposal is organized into three research areas. (1) Sample Acquisition, (2) Microfluidic Processing of the Oral Sample, and (3) Analyte Detection utilizing Up-converting Phosphor Technology (UPT). The fundamental UPT system exists, and has been used to quantitate drugs of abuse, antibodies to pathogens, and nucleic acids. We now present a plan to combine these separate analyses into a single system that can be used on-site to analyze up to 200 target analytes.