Towards development of eye-safe multiplex resonance raman device for point-of-care neurodiagnostics

为了改进创伤分诊和急救医学，该项目将重点探索和验证基于拉曼检测的基于拉曼检测的脑损伤指示性生化变化，这些变化来自于脑损伤后立即收集的生物液、脑组织培养物以及临床上收集的脑脊液(CSF)。由于视神经在眼后部可见，作为脑组织的投影，并沐浴在脑脊液中，它为了解大脑提供了一个光学清晰的窗口。因此，在该项目期间获得的关键知识将为开发一种新的眼睛安全的拉曼成像技术奠定平台，以检查视神经和筛选神经生物标志物，并允许监测药物输送，从而开发新的药物干预措施。该项目将推动朝着解决这一紧迫临床需求所需的长期方法迈出关键的发展步伐，开发能够在脑外伤检测中发挥直接作用的新一代PoC技术。对脑损伤进行快速、非侵入性的定量测试在必须快速决定患者是否可以安全地恢复全面活动的环境中尤其有用，例如，接触运动或在战场上暴露于冲击伤后。通过设计新的设备并对其进行测试，以检测生物体液和组织中的脑损伤标志物，以及监测脑损伤后的医疗干预和药物输送(通过快速、可靠、非侵入性和安全的方法检测和量化脑损伤)，该项目将提供医疗工具，以更好地做出治疗决策，改善脑损伤后的结果，从而提高生活质量，并最终提高存活率。使用激光光谱技术来非侵入性地检测、量化和监测脑外伤的治疗是非常新颖的，因此，该项目很可能在激光诊断应用、先进的光学设置、脑损伤和神经康复领域开辟新的天地。拉曼设备在未来可能会有广泛的相关应用，例如，在探索眼睛内的药物相互作用方面，无论是在新药开发中还是作为治疗的一部分。

Aiming at improving trauma triaging and point-of-care and emergency medicine, this project will focus on exploring and validating the Raman-based detection of TBI-indicative biochemical changes from biofluids, brain tissue cultures and in poartcilarly, the cerebrospinal fluid (CSF), collected immediately after TBI. Since the optic nerve is visible at the back-of-the-eye, as a projection of brain tissue and bathed in CSF, it provides an optically clear window into the brain. Therefore, the crucial knowledge acquired during this project, will lay the platform towards development of a novel eye-safe Raman imaging technique to examine the optic nerve and screen for neurological biomarkers as well as allow monitoring drug-delivery, towards developments of new pharmaceutical interventions. This project will enable crucial development steps towards the long-term approach required to address this urgent clinical need, developing a new generation of PoC technology capable of playing a direct role in TBI detection. The implications of having a rapid, non-invasive, quantitative test for TBI would be particularly useful in settings where rapid decisions have to be made regarding whether or not a patient is safe to return to full activity e.g., contact sports or after exposure to blast injury on the battlefield. Engineering novel device and testing it for detection of TBI-indicative markers from biofluids and tissues as well as for monitoring medical interventions and drug delivery post TBI (through rapid, reliable, non-invasive and safe methods of detecting and quantifying TBI), the project will deliver medical tools for better treatment decisions and improved outcomes subsequent to TBI for greater quality of life and ultimately, improved survival. The idea of using laser spectroscopic techniques to non-invasively detect, quantify and monitor treatment of TBI is highly novel and thus, it is likely that this project will break new ground in fields of diagnostic laser applications, advanced optical set-ups, brain-injury and neuro-rehabilitation. The Raman device could find a wide range of related applications in future e.g., in exploring drug interactions within the eye, either in new medicine development or as part of a treatment.