Electrochemical Measurement of S-nitrosothiols

描述（由申请人提供）：拟议的研究为体内和体外S-亚硝基硫醇（RSNO）传感器的商业开发提供了一种创新方法。选择性和敏感的RSNO传感器和相关仪器的设计将进行广泛的生物应用。与Cu（I）离子发生器和递送系统耦合的灵敏的一氧化氮电化学传感器的使用证明了在纳摩尔范围内的RSNO的检测。RSNO传感器将提供低分子量和高分子量（蛋白质）RSNO的优先检测。本提案将追求以下目标：（1）RSNO传感器，进一步与HPLC系统结合，以在线分离和检测不同的RSNO;（2）与RSNO传感器一起使用的电子控制器仪器的开发和商业化;（3）适用于生物采样的新型RSNO传感器的开发;（4）RSNO检测器和仪器的生物现场测试。这些目标将通过重要的电分析、生物分析开发和详细的生物学研究来实现。拟议的研究将利用该小组在NO纳米传感器，微芯片传感器和新型微混合器和Cu（I）发生器方面的最新进展。拟议项目的长期目标是创建商业上可行的和强大的仪器，以快速，选择性地测量S-亚硝基硫醇的浓度，并具有能够在生物相关浓度范围内检测这些物质的灵敏度。

DESCRIPTION (provided by applicant): The proposed research offers an innovative approach to the commercial development of in vivo and in vitro S-nitrosothiols (RSNO) sensors. The design of selective and sensitive RSNO sensors and related instrumentation will be undertaken for a wide range of biological applications. The use of sensitive nitric oxide electrochemical sensors coupled with a Cu(I) ion generator and delivery system, demonstrates detection of RSNOs in the nanomolar range. The RSNO sensors would offer preferential detection of both low and high molecular weight (protein) RSNOs. The following aims will be pursued in this proposal (1) the RSNO sensor, further combines with HPLC system to separate and detect different RSNOs on line 2) development and commercialization of an electronic controller instrument for use with RSNO sensors, (3) development of new RSNO sensors suitable for biological sampling (4) biological field testing of the RSNO detector and instrumentation. These goals will be reached with significant electroanalytical, bioanalytical development and detailed biological studies. The proposed research will capitalize upon the group's recent advances in NO nanosensors, microchip sensors, and a novel micromixer and Cu(I) generator. The long term goal of the proposed project is to create commercially viable and robust instrumentation with which to measure the concentration of S-nitrosothiols rapidly, selectively and with sensitivity capable of detecting these species in the biologically relevant concentration range.