Fluorescent Molecule Rotor for Blood Plasma Viscometry

描述（p由申请人提供）：目前液体复苏领域的结果表明，血浆粘度数据可能成为严重失血后容量恢复和血液替代的形式和类型决策过程中不可或缺的一部分。为此，所给液体的粘度及其对循环血液血浆粘度的影响是至关重要的。这些考虑与传统血浆扩张器的配方和人工血液替代液的新兴领域特别相关。迄今为止，等离子体粘度一直是通过机械装置来测量的，这种装置存在测量误差（高公差），耗时，需要相当大的液体量，并且需要细致的清洗。分子转子是一类新型荧光分子，显示出粘度依赖的荧光量子产率，因此可以用来确定其环境的粘度。根据我们发表的研究结果，分子转子可以成功地用于探测离体血浆粘度。在此，我们提出了荧光探针优化血浆粘度测量的发展。这种测量流体粘度的新技术将允许对血浆和血浆扩张器的粘度进行快速和低样本量的测量。一旦优化，粘度荧光传感器将附着在固体光学表面上，为固体结合光学分子粘度计的开发提供关键和独特的组件。我们期望这种测量流体粘度的全新技术将在广泛的医学诊断方法中得到应用，因为现有方法普遍基于机械/液压机械的复杂性，目前还无法考虑到这一点。此外，该技术还有可能应用于许多其他需要在过程中直接实时监测粘度的领域。在生物医学应用方面，该技术的独特之处在于它解决了迄今为止生物医学粘度测量不切实际的所有问题。它利用小体积样品，大小相同的必要的微红细胞压积测定。它测量等离子体粘度。它使用廉价的一次性样品管，因此不需要清洗粘度表面和机械校准。这是一项需要最少培训和人员的技术。这是一种新的测量方法，在一个廉价和实用的系统中实现，配置用于产生血浆粘度的临床相关数据，实验证明这对预测生存至关重要。它允许以最少的资源部署对大量人口进行测试。这项技术可以将血浆粘度作为临床相关参数进行处理，并通过一种简单而明确的血细胞比容测定方法开辟了新的医学视野。

DESCRIPTION (p rovided by applicant): Current results in the field of fluid resuscitation indicate that data on plasma viscosity may become an integral part of the decision making process on the form and type of volume restitution and blood replacement following severe blood losses. To this end, the viscosity of the fluid to be administered and its effect on the resulting plasma viscosity of the circulating blood is of paramount importance. These considerations are especially relevant to the formulation of conventional plasma expanders and the emergent field of artificial blood replacement fluids. To date, plasma viscosity has been measured by mechanical devices, which are subject to measurement errors (high tolerances), are time-consuming, requires a fairly large amount of fluid and require meticulous cleaning. Molecular rotors are a novel class of fluorescent molecules that show a viscosity-dependent fluorescence quantum yield and thus can be exploited to determine the viscosity of their environment. Based on our published findings, molecular rotors can be successfully used to probe blood plasma viscosity ex-vivo. Herein we propose the development of fluorescent probes optimized for blood plasma viscosity measurements. This new technology for measuring fluid viscosity will allow fast and low sample volume measurements of the viscosity of blood plasma and blood plasma expanders. Once optimized, the viscosity fluorosensors will be attached to a solid optical surface to generate the critical and unique component for the development of solid-bound optical molecular viscometers. We expect that this fundamentally new technology for measuring fluid viscosity will have applications in a broad range of medical diagnostic approaches, which to the present could not be contemplated because of the complexity of existing methods universally based on mechano/hydraulically based machinery. Furthermore, this technology has the potential to be applicable in many other fields where direct real-time monitoring of viscosity during a process is required. In terms of biomedical applications the uniqueness of the technology proposed is that it addresses all the issues that so far have biomedical viscosity measurements impractical. It utilizes small volume samples, of size identical to that necessary for microhematocrit determinations. It measures plasma viscosity. It uses inexpensive, disposable sample tubes, and therefore does not require cleaning of viscometric surfaces and mechanical calibration. It is a technology that requires minimal training and personnel. It is a new measuring method, implemented in an inexpensive and practical system, configured to yield clinically relevant data on plasma viscosity that has been experimentally demonstrated to be critical for predicting survival. It allows testing of large populations with minimal deployment of resources. This technology allows dealing with plasma viscosity as a clinically relevant parameter, and opens a new medical horizon by means of a procedure that is a simple and unequivocal as a hematocrit determination.