Microcarriers designed for protein & sera free media

描述（由申请人提供）：本研究的总体目标是开发一种微载体，该微载体包被在I型胶原中发现的合成的15个氨基酸肽序列。这种微载体将取代目前使用天然胶原蛋白或明胶的微载体，在要求无动物产品的条件下。目前，三种明胶包膜微载体，一种是明胶连接葡聚糖（Cytodex III，由英国Amersham-Pharmacia生物技术公司制造），另一种是Solohill工程公司的两种明胶包膜聚苯乙烯微载体，是目前在工业规模生产锚定依赖性细胞中使用最广泛的微载体基质。随着病毒疫苗和其他生物制品的制造商逐渐转向完全不含动物产品的生产条件，含有胶原蛋白或明胶的底物将不可接受。合成胶原肽包被的微载体是一种理想的替代品。

DESCRIPTION (provided by applicant): The overall goal of this study is to develop a microcarrier coated with a synthetic, 15 amino acid peptide sequence found in type I collagen. This microcarrier would replace current microcarriers utilizing native collagen or gelatin in protocols requiring animal product-free conditions. Currently three gelatin-coated microcarriers, a gelatin-linked dextran (Cytodex III, made by the United Kingdoms's Amersham-Pharmacia Biotechnology) and Solohill Engineering's two gelatin-coated polystyrene microcarriers, are the most extensively used microcarrier substrates in the industrial scale production of anchorage-dependent cells today. As manufacturers of viral vaccines and other biologicals move toward completely animal product-free manufacturing conditions, a substrate containing collagen or gelatin will not be acceptable. The synthetic-collagen-peptide coated microcarriers would provide an ideal replacement. In the Phase I portion of this SBIR grant application, we propose the following experimental program to demonstrate the feasibility of producing such a product. Specific Aim I. To attach a synthetic-collagen-peptide to the surface of polystyrene microcarrier core beads and to compare the synthetic peptide - coated microcarriers with currently available collagen-coated polystyrene microcarriers for ability to support attachment and growth of anchorage-dependent animal cells under serum-free conditions. Specific Aim II. To attach a synthetic-collagen-peptide to the surface of charged-surface polystyrene microcarrier core beads (Hillex microcarriers) and to compare the synthetic peptide-coated microcarriers with currently available charged-surface polystyrene microcarriers for ability to support attachment and growth of anchorage-dependent animals cells under serum-free conditions. Specific Aim III. To attach a synthetic-collagen-peptide to the surface of dextran microcarrier core beads and to compare the synthetic peptide-coated microcarriers with currently-available collagen-coated dextran microcarriers for ability to support attachment and growth of anchorage-dependent animals cells under serum-free conditions.