Development of Novel Robust Laundry Enzymes for Cold Wash Applications by both rational and directed evolution and using micro-fluidic approaches

该项目将参与从嗜极端细菌和古细菌中鉴定新型酶，这些酶具有改进的特性，可用于洗涤剂和医疗保健领域。特别是对于可持续的冷水洗涤来说既强大又具有高活性的酶。以前的研究使用了从嗜冷细菌中分离出来的酶。这种方法有其局限性，因为尽管酶在低温下是有活性的，但它们对于洗衣目的来说不够稳健。该方法将涉及使用一个强大的酶支架与一个灵活的活性位点，将允许在环境温度下的高活性。这种方法将通过理性和定向进化以及使用微流体方法来进行。

The project will be involved in identifying novel enzymes from extremophilic bacteria and archaea that have improved properties for use in the detergent and healthcare sector. Specifically enzymes that are both robust and have high activity for sustainable cold water washing. Previous studies have used enzymes isolated from psychrophilic bacteria. This approach has its limitations since although the enzymes are active at low temperatures they are not robust enough for laundry purposes. The approach will involve using a robust enzyme scaffold together with a flexible active site that will allow high activity at ambient temperatures. This approach will be carried out by both rational and directed evolution and using micro-fluidic approaches.