Interrogating the RAS-RAF-MEK-ERK pathway using Protein Interference.

这个多学科项目将采用合成生物学筛选，以确定新的调节剂/抑制剂的RAS-RAF-MEK-ERK途径，一个关键的细胞命运信号通路，使用PhoreMost的蛋白质干扰（PROTEIN-i）筛选技术。ERK途径通常通过ERK催化的促凋亡蛋白BIMEL的磷酸化促进细胞存活。短形式的BIM（BIM）缺乏这些磷酸化位点。这提供了利用BIMS作为ERK驱动的毒性基因的机会，为蛋白质-I探针提供表型选择。

This multi-disciplinary project will employ synthetic biology screens to identify novel regulators/inhibitors of the RAS-RAF-MEK-ERK pathway, a critical cell fate signalling pathway, using PhoreMost's protein interference (PROTEIN-i) screening technology. The ERK pathway normally promotes cell survival through ERK-catalysed phosphorylation of the pro-apoptotic protein BIMEL. The short form of BIM (BIMS) lacks these phosphorylation sites. This provides an opportunity to harness BIMS as an ERK-driven toxic gene, providing phenotypic selection for PROTEIN-I probes.