Pathogen-induced changes in plant-insect interactions for crop and ornamental species in the genus Solanum

主题：农业和食品安全将研究疾病对植物-昆虫相互作用的影响，使用英国花园中存在的茄子物种：西红柿、土豆、茄子，以及观赏植物龙葵、疏松茄子、龙葵等。将被检查的病毒病原体将是我在2015年夏天对英国花园中影响茄属植物的病毒进行的调查中确定的最主要的病毒。目前正在对样本进行分析，但这些感染茄子的病毒很可能是马铃薯Y病毒和巨细胞病毒(由蚜虫传播)的分离物，以及烟草花叶病毒(烟草花叶病毒和番茄花叶病毒)和马铃薯X病毒(不由蚜虫传播)的分离物。我将研究选定的病毒对咀嚼昆虫Manduca sexta的幼虫的定殖性、取食行为、存活和表现的影响，这两种病毒都能传播各种病毒。蜜蜂对健康和患病植物发出的视觉和嗅觉提示的反应将使用自由选择和条件分析进行调查。昆虫的反应将与植物挥发性排放(使用GC-MS)和植物可溶性因子(例如使用LC-MS在花蜜中)的变化相关联。

Theme: Agriculture and Food SecurityI will examine the effects of disease on plant-insect interactions using Solanum species present in UK gardens: tomato; potato; aubergine, and the ornamentals Solanum crispum, S. laxum, S. aviculare and others. The viral pathogens to be examined will be those most predominant identified by my survey of viruses affecting Solanum species in UK gardens undertaken in the summer of 2015. The samples are currently being analysed but these Solanum-infecting viruses are likely to be isolates of Potato virus Y and CMV (aphid-transmitted), as well as tobamoviruses (Tobacco mosaic virus and Tomato mosaic virus) and Potato virus X (not aphid transmitted). I will examine the effects of the selected viruses on colonisation, feeding behaviour, survival and performance of larva of the chewing insect Manduca sexta and the aphids Myzus persicae (a non-specialist) and Macrosiphum euphorbiae (Solanum specialist), both of which vector a variety of viruses. Bee responses to visual and olfactory cues emitted by healthy versus diseased plants will be investigated using free choice and conditioning assays. Insect responses will be correlated with changes in plant volatile emission (Using GC-MS) and in plant soluble factors e.g. in nectar using LC-MS.