Cell Identity and differentiation in stimulus response

描述(申请人提供)：器官是由具有不同身份的细胞组成的，旨在以专门的方式解释刺激并协调完成复杂的功能。为了了解细胞如何执行这些特殊的功能，我计划研究细胞特性改变刺激反应的质量和数量的机制。我建议使用荧光激活细胞分选(FACS)结合细胞类型特异的荧光标记系来分离在刺激处理后的根的单个细胞类型。使用微阵列，然后我将分析在根的每种细胞类型中发生的转录变化，并识别展示细胞类型特定转录反应的基因。这些数据不仅将有助于更好地了解细胞特性与刺激感知和反应之间的关系，而且还将导致开发监测这种调控的标记和基于标记的突变筛选，以确定控制这种调控的遗传机制。此外，我建议合作开发一个用于可视化根系发育和生理的高通量平台，称为“根阵列”，它将促进这里提出的工作，并为未来实验的设计做出贡献。

DESCRIPTION (provided by applicant): Organs are composed of cells with distinct identities designed to interpret stimuli in specialized ways and coordinately fulfill complex functions. In order to understand how cells perform these specialized functions I plan to study the mechanisms by which cell identity alters the quality and quantity of a stimulus response. I propose to use Fluorescent Activated Cell Sorting (FACS) in combination with cell-type specific fluorescent marker lines to isolate individual cell types of the root after treatment with a stimulus. Using microarrays, I will then analyze the transcriptional changes that occur in each cell type of the root and identify genes that demonstrate cell-type specific transcriptional responses. These data will not only lead to a better understanding of the relationship between cell identity and stimulus perception and response but will also lead to the development of markers to monitor this regulation and marker-based mutant screens to identify the genetic mechanisms that control it. In addition, I propose to collaborate in the development of a high-throughput platform for visualizing root development and physiology termed the "root array" that will facilitate the work proposed here and contribute to the design of future experiments.