GENETIC MAPPING RESOURCE FOR COMMON MAMMALIAN DISEASES

超出所提供的空间。伴侣动物的特征是可遗传的人类疾病，许多没有老鼠对应。为了弥合人类和老鼠之间的差距，基因组计划已经启动了伴侣动物，特别是猫和狗。针对这些物种的生物学重点基因组计划将更有效地跨越老鼠和人类之间的差距。有针对性的基因图谱方法将弥补这些举措规模较小的问题，并允许伴侣动物的基因组为人类健康和科学进步揭开其生物学秘密，为世界各地的研究人员提供宝贵的工具和资源。我们的长期目标是利用伴侣动物的遗传疾病的知识，以深入了解类似疾病的发病机制，在man.Objective本申请是开发比较的猫和狗的遗传图谱，通过使用一个集中的方法映射500个基因，已知具有生物相关性，并确定300个微卫星标记与这些基因。这一重点将提供资源，并促进人类和伴侣动物研究人员的研究，他们对特定的生物过程，简单或复杂的特征以及遗传或获得性疾病感兴趣。我们将通过三个具体目标来完成这项任务：1）开发约500个基因的保守引物并筛选变异。2)确定每个基因的物种特异性BAG克隆，以确定基因相关的微卫星。3)基因变异和基因相关微卫星将在适当的作图资源中进行基因分型。由于该项目将生成针对人类难以研究的疾病的比较地图，人类健康将迅速受益于伴侣动物的生物学。在动物和人类中研究这些疾病的研究人员将在资源方面取得飞跃，包括候选基因、基于家族和群体的关联的连锁标记以及大型插入基因组BAG克隆。这项拟议中的研究意义重大，因为它将在三个物种中提供一个与人类相当的框架图，这些物种具有困扰人类的复杂和简单的疾病特征。性能现场=

EXCEED THE SPACE PROVIDED. Companion animals have been characterized for heritable human diseases, many do not have a mouse counterpart. To bridge the gap between human and mouse biology, genome projects have initiated for companion animals, particularly for the cat and the dog.Biologically focused genome initiatives for these species would span the gap between mouse and man more efficiently. A focused gene mapping approach would remedy the smaller scale of these initiatives and allow the genomes of companion animals to unlock their biological secrets for human health and scientific advancement, providing an invaluable tool and resources to researchers throughout the world. Our long-range goal is to use knowledge of genetic diseases in companion animals to gain insight into the pathogenesis of comparable diseases in man. The objective of this application is to develop comparative genetic maps of the cat and dog by using a focused approach of mapping 500 genes, known to have biological relevance, and to identify 300 microsatellite markers associated with these genes. This focus will provide resources and facilitate the research of human and companion animal investigators who have interests in particular biological processes, simple or complex traits and inherited or acquired diseases. We will accomplish this task through three specific aims: 1) Develop conserved primers for approximately 500 genes and screen for variation. 2) Identify species-specific BAG clones for each gene to identify gene-associated microsatellites. 3) Gene variations and the gene-associated microsatellites will be genotyped in the appropriate mapping resources. Because this project will generate comparative maps focusing on diseases that are difficult to study in humans, human health will rapidly benefit from the biology of companion animals. Researchers studying these diseases in animals and humans will have a leap in resources, including candidate genes, linked markers .for familial and population based associations, and large insert genomic BAG clones. The proposed research is significant, because it will provide a framework map,comparable to humans, in three species that have complex and simple disease traits that plague humans. PERFORMANCE SITE ========================================Section End===========================================