Core--Skin Cell Culture

描述(申请人提供)：皮肤细胞培养核心(SCCC)旨在促进 UAB全校皮肤病研究中心(SDRC)成员在皮肤细胞生物学方面的跨学科研究。SCCC将提供从小鼠或人类皮肤中产生和维持表皮和真皮细胞特异性培养的专业知识。此外，它还将提供开发相当于木筏皮肤的培养物的专业知识。该中心的具体目标是：以高效率、高成本效益的方式向中心成员提供各种表皮和真皮细胞类型的中心来源；2)维持质量控制措施，以确保无污染培养；3)提供专门的细胞培养系统以及人和小鼠细胞的转基因方面的专业知识；4)开发混合培养方法，以产生漂浮角质形成细胞与朗格汉斯细胞和/或黑素细胞的混合共培养，以满足国家发改委成员的兴趣和需求。

DESCRIPTION (provided by applicant): The Skin Cell Culture Core (SCCC) aims to promote interdisciplinary research in skin cell biology among UAB campus-wide Skin Diseases Research Center (SDRC) members. The SCCC will provide expertise in the generation and maintenance of epidermal and dermal cell specific cultures from either mouse or human skin. In addition, it will provide expertise in the development of RAFT skin equivalent cultures. The specific aims of the core are l) to provide a central source of epidermal and dermal cell types to Center members in an efficient cost effective manner, 2) to maintain quality control measures to ensure contamination-free cultures, 3) to provide expertise in specialized cell culture systems and transfection for both human and mouse cells and 4) to develop methods for generating mixed co-cultures of raft keratinocytes with Langerhans cells and/or melanocytes to anticipate increased interest and demand by SDRC members.