CORE--DE PEDIATRIC COBRE: CELL SCIENCE CORE

细胞科学核心对这个拟建中心的所有五个项目的发展至关重要。核心汇集了系内所有可用的细胞和分子生物学资源，并提供对特拉华大学和特拉华生物技术学院提供的其他服务的访问。在CORE内部，将建立一个准备设施，以获取用于DNA、RNA和蛋白质分离的人体组织。还将建立原代人类细胞培养，以支持研究人员的项目。这些组织和细胞将通过临床服务核心(核心B)获得，以确保患者的机密性并遵守联邦、州和机构的法规。制备核心将创造EB病毒永生化的淋巴母细胞，以及来自皮肤和其他组织的原代成纤维细胞。将建立一个分析设施，由细胞生物学资源、蛋白质生物化学组成 资源和分子生物学分核心。细胞生物学资源将集中仪器的使用和可用性，并提供细胞科学方面的操作和实验专业知识。这些资源将包括共聚焦显微镜、荧光和可见光光谱以及FACS。通过与特拉华州生物技术研究所的协议，多光子共聚焦显微镜、透射式和扫描电子显微镜以及原子力显微镜也将可用。蛋白质生物化学资源将汇集蛋白质表达、纯化和表征方面的设备和专业知识。将开发二维凝胶分析系统，并与大学达成协议。特拉华州大学将通过MALDI/TOF/TOF和各种替代的质谱学技术提供识别蛋白质的扩展能力。 分子生物学分核心将提供一系列用于DNA测序和遗传分析的现代分子技术并提供专业知识。这些技术包括焦磷酸测序、变性高效液相色谱突变分析和实时聚合酶链式反应。创建一个统一的细胞科学核心将提供一个独特的设施，为每个研究人员提供访问多种现代实验方法的途径。核心的经验丰富的调查员将主动与项目调查员互动，以加强项目开发。

A Cell Science Core is critical to the development of all five of the projects in this proposed Center. The Core brings together all of the cell and molecular biological resources available within the department, and provides access to additional services available at the University of Delaware and the Delaware Biotechnology inst. Within the Core, a preparative facility will be established to procure human tissues for DNA, RNA, and protein isolation. Primary human cell cultures will also be established to support investigators' projects. The tissues and cells will be obtained through the Clinical Services Core (Core B) to ensure patient confidentiality and compliance with federal, state and institutional regulations. The preparative core will create EBV-immortalized lymphoblasts, and primary fibroblasts from skin and other tissues. An Analytical Facility will be created, consisting of a Cell Biology Resource, a Protein Biochemistry Resource, and a Molecular Biology Sub-Core. The Cell Biology Resource will centralize use and availability of instrumentation and provide operational and experimental expertise in cell science. The resources will include Confocal microscopy, fluorescence and visible spectroscopy, and FACS. Through an agreement with the Delaware Biotechnology Inst., multiphoton confocal microscopy, trasmission and scanning electron microscopy, and atomic force microscopy will also be available. The Protein Biochemistry Resource will bring together equipment and expertise for protein expression, purification, and characterization. 2D gel analysis systems will be developed, and an agreement with the Univ. of Delaware will provide extended capabilities to identify proteins by MALDI /TOF/TOF and a variety of altemative mass spectrometric techniques. The Molecular Biology Sub-Core will provide access to and expertise in a range of modem molecular techniques for DNA sequencing and genetic analysis. These techniques include pyrosequencing, mutation analysis by denaturing high performance liquid chromatography, and real-time PCR. The creation of a unified Cell Science Core wilt provide a unique facility to provide each investigator access to multiple modern experimental approaches. The experienced investigators in the Core will interact proactively with the project investigators to enhance project development.