Capillary DNA Sequencers for the OUHSC Core Facility

描述（由申请人提供）：俄克拉荷马州大学健康科学中心（OUHSC）微生物基因组学实验室设有一个核心DNA测序设施，该设施是大学建立的服务单位，负责为OUHSC社区提供DNA测序、HLA分型、高通量寡核苷酸合成、微阵列制造、杂交和分析以及生物信息学支持。在行政上，这一核心设施设在微生物学和免疫学系，由约翰·扬多洛博士主持;该部门负责核心设施的预算和信托监督。对该核心设施的机构承诺始于1995年，当时OUHSC承诺通过购买三台Pharmacia ALF Express平板凝胶DNA测序仪（目前用于HLA分型），与最初建立核心设施的NSF设备赠款匹配50%。在此期间，该机构为购买两台ABI 377平板凝胶测序仪、一台ABI 3700毛细管DNA测序仪提供了支持，为第二台ABI 3700提供了部分支持，并为购买其他设备提供了额外资金，包括一台带有RAID阵列和外部磁带备份的Sun Enterprise 450服务器、一台Genomic Solutions Flexsys阵列打印机/菌落拾取机器人、并为生物自动化MerMade多重寡核苷酸合成器提供配套资金。随着其他NIH和NSF赠款和OUHSC多年来提供的额外资金，该大学已投资超过100万美元用于该核心设施的资本设备。由于该设施的成功，我们校园对DNA测序服务的需求已经增长到现有设施在没有额外设备的情况下无法继续支持这一需求的程度。具体而言，我们建议购买：1）ABI 3100 16-毛细管DNA测序仪，用于取代ABI 377平板凝胶测序仪，提高通量，降低成本，提高数据质量，并缓解测序服务中现有的瓶颈，该瓶颈减缓了小型项目的DNA测序，以及2）ABI 3730 48-毛细管DNA测序仪，替换原来的Pharmacia ALF快速测序仪，将我们的HLA分型能力升级为毛细管方法，提高了通量并降低了成本。

DESCRIPTION (provided by applicant): The Oklahoma University Health Sciences Center (OUHSC) Laboratory for Microbial Genomics houses a core DNA sequencing facility that is a university-established service unit, responsible for providing DNA sequencing, HLA typing, high-throughput oligonucleotide synthesis, microarray fabrication, hybridization and analysis, and bioinformatics support to the OUHSC community. Administratively, this core facility is housed within the Department of Microbiology and Immunology, chaired by Dr. John Iandolo; the department has the responsibility for budgetary and fiduciary oversight of the core facility. The institutional commitment to this core facility began in 1995, when OUHSC committed a 50% match to an NSF equipment grant that originally established the core facility, by the purchase of three Pharmacia ALF Express slab gel DNA sequencers, which are currently used for HLA typing. In the intervening years, the institution has provided support for the purchase of two ABI 377 slab gel sequencers, one ABI 3700 capillary DNA sequencer, partial support for a second ABI 3700, and additional funds for the purchase of other equipment including a Sun Enterprise 450 server with RAID array and external tape backup, a Genomic Solutions Flexsys array printer/colony picker robot, and matching funds for a Bioautomation MerMade multiplex oligonucleotide synthesizer. With other NIH and NSF grants and additional funds provided by OUHSC over the years, the university has invested well over $1 million in capital equipment for this core facility. Because of the success of this facility, the demand for DNA sequencing services on our campus has grown to the point where the existing facility cannot continue to support this demand without additional equipment. Specifically, we propose to purchase: 1) an ABI3100 16-capillary DNA sequencer to replace the ABI377 slab gel sequencers, increase throughput, cut costs, enhance data quality, and relieve the existing bottleneck on sequencing service that slows DNA sequencing for small projects, and 2) an ABI3730 48-capillary DNA sequencer, replacing the original Pharmacia ALF express sequencers to upgrade our HLA typing capacity to capillary methodology, increasing throughput and cutting costs.