CORE--GENOMICS AND CHEMICAL SYNTHESIS CORE

核心C：膳食、基因组学和化学合成 博士Helferich将担任该核心的资源领导人，并将监督饮食子核心。Benita Katzenellenbogen博士将监督基因组学子核心，John Katzenellenbogen博士将监督化学合成子核心。 饮食子核心将提供项目1-3中使用的所有饮食。总的来说，这些饮食 将基于半纯化的无大豆饮食（AIN-93 G）。该子核心还将提供 染料木黄酮和雌马酚，以及用于动物的任何其它膳食雌激素 研究项目。Helferich博士将与每个项目负责人密切合作， 在每项研究中使用适当的饮食。基因组学子核心将提供基因表达 利用微阵列技术，对所有的研究项目进行分析。微阵列表达数据将被确认，并使用定量PCR方法与专用仪器在这个子核心进一步量化。该子核心还将直接或通过与校园生物统计学专家和咨询服务的安排，提供微阵列数据的统计分析。化学合成子核心将提供拟用于项目1-3中拟定的实验室动物研究的植物雌激素或代谢物的合成或化学修饰，并与核心B一起确保这些组分的同一性和纯度。 通过集中这些对项目1-4至关重要的活动，我们将确保在项目1-4中进行的研究使用一套通用的化合物、无植物雌激素基础饮食和使用标准化方案的基因组分析方法。

Core C: Dietary, Genomics, and Chemical Synthesis Dr. Helferich will serve as the resource leader for this Core and will oversee the Dietary Subcore. Dr. Benita Katzenellenbogen will supervise the Genomics Subcore, and Dr. John Katzenellenbogen will supervise the Chemical Synthesis Subcore. The Dietary Subcore will provide all of the diets to be used in Projects 1-3. In general, these diets will be based on a semi-purified, soybean-free diet (AIN-93G). This Subcore will also provide genistein and equol, as well as any other dietary estrogens that are to be used in the animal research projects. Dr. Helferich will work closely with each project leader to establish the most appropriate diets to use in each study. The Genomics Subcore will provide gene expression profiling for all the research projects, using microarray techniques. Microarray expression data will be confirmed and further quantified using quantitative-PCR methods with dedicated instrumentation in this Subcore. This Subcore will also provide statistical analysis of microarray data, either directly or through arrangements with campus biostatistics experts and consulting services. The Chemical Synthesis Subcore will provide synthesis or chemical modifications of the phytoestrogens or metabolites to be used in the laboratory animal studies proposed in Projects 1-3, and together with Core B will assure the identity and purity of these components. By centralizing these activities critical to Projects 1-4, we will assure that the research conducted in projects 1-4 utilize a common set of chemical compounds, phytoestrogen-free base diets, and genomic analysis methods using standardized protocols.