Washington University Center for Translational Neuroscience

将基础神经生物学的发现从啮齿动物模型转化到人类身上的能力需要谨慎 表型鉴定。许多，如果不是全部，人类神经精神障碍涉及重大的变化 行为、感觉运动加工和突触功能。虽然在发电方面取得了很大的进展 神经精神障碍的啮齿动物模型，包括转基因小鼠的遗传模型， 在行为和突触水平上系统地描述这些模型是滞后的，而且往往不是 被整合到各个科学学科中。我们建议创建功能评估核心(FAC) 这将解决这些问题，并促进更广泛的华盛顿大学(吴)的翻译研究 神经科学界。这一核心将利用几个吴部门的现有专门知识，包括 精神病学、神经学、耳鼻喉科和麻醉学，并将提供啮齿动物行为的分析， 感觉功能和疼痛处理结合体内和体外突触功能的研究。 FAC的具体目标是：1.评估啮齿动物的行为，包括学习和 记忆、情绪性、感觉运动能力和社会互动、疼痛相关行为和感觉 2.使用生理学方法评估啮齿动物模型的突触功能 范围从体内脑电和诱发电位研究到体外脑片准备 和分离细胞培养中的单突触研究；以及3.提供科学专业知识和 促进轻松获取功能评估研究所需的管理技能 维持一个高效率的核心设施来开展这项工作。与此目标相关的基于客户端的服务 包括在设计研究、提出和解释数据、进行适当 统计分析，以及数据库管理、访问和存档中与 行政和信息学核心。FAC旨在充当目标发现之间的桥梁 和特征(通过分子分析、光学成像和病毒载体核心)和翻译 在生物医学和临床数据获取核心方面的努力。反过来，外交事务委员会将成为 与吴的其他现有翻译研究努力相结合，包括神经成像方面的项目， 人类神经遗传学和神经临床试验。

The ability to translate discoveries in basic neurobiology from rodent models to humans requires careful phenotypic assessment. Many, if not all, human neuropsychiatric disorders involve significant changes in behavior, sensorimotor processing and synaptic function. While great progress is being made in generating rodent models of neuropsychiatric disorders, including genetic models in transgenic mice, the ability to characterize these models systematically at behavioral and synaptic levels has lagged and has often not been integrated across scientific disciplines. We propose to create a Functional Assessment Core (FAC) that will address these issues and facilitate translational research in the broader Washington University (WU) neuroscience community. This core will draw upon existing expertise in several WU departments, including Psychiatry, Neurology, Otolaryngology and Anesthesiology, and will provide analyses of rodent behavior, sensory function and pain processing in combination with in vivo and in vitro studies of synaptic function. The specific aims of the FAC are: 1. To provide assessment of rodent behavior, including learning and memory, emotionality, sensorimotor capabilities and social interactions, pain-related behaviors, and sensory function; 2. To provide assessment of synaptic function in rodent models using physiological approaches that range from in vivo electroencephalography and evoked potential studies to in vitro brain slice preparations and single-synapse studies in dissociated cell culture; and 3. To provide scientific expertise and management skills necessary for promoting easy access to functional assessment research and for maintaining an efficient core facility to carry out such work. Client-based services associated with this aim include consultation in designing studies, in presenting and interpreting data, in conducting appropriate statistical analyses, and in database management, access, and archiving in association with the Administrative and Informatics Cores. The FAC is designed to serve as a bridge between target discovery and characterization (via the Molecular Analysis, Optical Imaging and Viral Vector Cores) and translational efforts in the Biospecimen and Clinical Data Acquisition Core. In turn, the FAC will serve as a conduit for integration with other existing translational research efforts at WU including programs in neuroimaging, human neurogenetics and neuroclinical trials.