Stimulated Raman Scattering to track the delivery of ASO and/or TPD into cells and tissues

反义寡核苷酸（ASO）是一种新的治疗方式，已迅速获得牵引力，目前约有120个ASO在临床试验和6个批准的药物上市。ASO是短的、合成的、化学修饰的寡核苷酸，其结合靶RNA的互补序列，诱导其降解或剪接过程的改变。尽管它们在临床上取得了惊人的成功，但细胞摄取阿索的内吞机制仍然知之甚少，因此限制了阿索的功效。因此，提高我们对ASO如何在细胞和组织中被吸收和分布的理解将有助于加速该领域的药物发现。第二种新兴的治疗策略是通过劫持细胞内蛋白质水解机制来诱导细胞靶标的靶向蛋白质降解（TPD）。本研究的目的是基于受激拉曼散射（SRS）显微镜的最新创新，开发一种在亚细胞分辨率下以高灵敏度和特异性跟踪细胞和组织中阿索和/或TPD治疗剂的递送和分布的方法。

Antisense oligonucleotides (ASOs) are a new therapeutic modality that have rapidly gained traction, with about 120 ASOs currently in clinical trials and 6 approved drugs on the market. ASOs are short, synthetic, chemically modified oligonucleotides that bind to the complementary sequence of the target RNA, inducing its degradation or alteration of splicing process. Despite their phenomenal success in the clinic, the endocytic mechanisms of ASO uptake by cells are still poorly understood thus limiting ASO efficacy. Therefore, improving our understanding of how ASOs are taken up and distributed in cells and tissues would help accelerate drug discovery in this field. A second emerging therapeutic strategy is the induction of Targeted Protein Degradation (TPD) of cellular targets by hijacking the intracellular proteolysis machinery. The aim of this study is to develop a method to track the delivery and distribution of ASO and/or TPD therapeutics in cells and tissues with high sensitivity and specificity at subcellular resolution based on the newest innovations in Stimulated Raman Scattering (SRS) microscopy.