AREA IV IMMUNOLOGY

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。免疫学第四领域的目标是了解控制免疫系统发育和功能的细胞与细胞之间的相互作用和分子机制。在该小组中，一个较小的小组专注于在发育和调节水平上调节自身免疫的机制。第四区有6名成员，包括来自纽约大学医学院微生物学和免疫学系的2名研究活跃教师和生物系的4名研究活跃教师。威廉·博托、舒巴·戈文德和曾傑瑞·盖登是直接通过RCMI拨款聘用的。马克·佩扎诺在担任RCMI项目副主任并与盖登博士在研究项目上合作了几年后，被聘为生物系的教员。Paul Gottlieb和Linda Spatz被纽约州立大学医学院聘用，并被招募加入RCMI计划，因为当我们在上一份提案中启动免疫学小组时，他们积极开展专注于自身免疫的研究合作。所有这些研究人员目前都有外部资金用于他们的研究项目，许多人在集团内部和与外部调查人员都有合作项目。该小组的研究兴趣广泛，从研究果蝇的先天性免疫系统发育和功能，到KSHV在人类的传播和进化。我们有几个研究人员，他们研究B细胞和T细胞中免疫细胞的发育以及免疫系统的调节，他们专注于免疫学中的一个关键问题，即免疫系统如何区分自我和非我。这些研究与自身免疫性疾病的发展直接相关。在这项申请中，我们正在申请资金，以聘请一名额外的免疫学家，以增加我们在这一令人兴奋的研究领域的临界质量。这个人将专注于脊椎动物先天免疫的研究，以最好地促进与我们研究小组现有成员的合作，并在近年来经历了新兴趣革命的领域增加临界质量。第四区的设备申请包括一个新的荧光激活细胞分类器，以取代在第一轮RCMI资金中购买的现已不起作用但利用率很高的Coulter EPICS Elite。这种设备虽然对免疫学家的研究工作不可或缺，但对RCMI计划其他领域的许多研究人员来说也是一个非常重要的工具。对于我们的中央设施，我们正在申请资金，用于更换在第一个授权期内购买的不能再使用的物品，供应商也不会继续为它们提供服务。我们需要更换两台用于我们中央设施设备室的冷水机组，以及一台高压灭菌器。我们需要增加两台？80 Revco冷冻机来更换旧设备，并为新员工提供额外的存储空间。我们需要更换我们的分光光度计(1988年购买)，一个SPEEDVAC，以及三个RO和MILI Q系统(都是1987年购买的)。我们还申请资金购买我们的DNA测序仪BD LSR II FACS分析仪以及本建议书中要求的新细胞分类器和共聚焦显微镜的服务合同。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of Area IV, Immunology, is to understand the cell-to-cell interactions and molecular mechanisms that control the development and function of the immune system. Within the group, a smaller subgroup is focused on the mechanisms that regulate autoimmunity, both at the developmental and regulatory levels. There are six members of Area IV, consisting of 2 research active faculty from the CUNY Medical School department of Microbiology and Immunology and 4 from the department of Biology. William Boto, Shubha Govind and Jerry Guyden were hired through the RCMI grant directly. Mark Pezzano was recruited for a faculty position in the Biology department, after serving as Deputy Director of the RCMI program and collaborating on research projects with Dr. Guyden for several years. Paul Gottlieb and Linda Spatz were hired by the CUNY Medical School and recruited to join the RCMI program because of their active research collaboration focused on autoimmunity when the immunology subgroup was initiated in our last proposal. All of these researchers currently have outside funding for their research projects and many have collaborative projects both within the group and with outside investigators. The research interests of the group are diverse, ranging from studies of innate immune system development and function in Drosophila to KSHV transmission and evolution in humans. We have several investigators who study aspects of immune cell development in both B and T cells and immune system regulation, which are focused on a key question in Immunology as to how the immune system distinguishes self from non-self. These studies are directly relevant to the development of autoimmune diseases. In this application, we are requesting funds to hire one additional immunologist to increase our critical mass in this exciting area of research. This individual will have a research focus in innate immunity in vertebrates, to best foster collaboration with the existing members of our research group and to increase the critical mass in an area that has experienced a revolution in new interest in recent years. The equipment requests for Area IV include a new fluorescence activated cell sorter, to replace the now non-functional but heavily utilized Coulter Epics Elite purchased in the initial round of RCMI funding. This equipment while indispensable to the research efforts of the Immunologists is also a very important tool for many researchers in other areas of the RCMI program. For our central facilities we are requesting funds for replacement of items purchased during the first granting period that can no longer be used nor will the vendors continue to service them. We need to replace two Cold wave chilling units, used for our central facility equipment rooms, as well as an autoclave. We need to add two ¿80 Revco freezers to replace old equipment and provide additional storage space for new hires. We need to replace our Spectrophotometer (purchased in 1988), a speedvac, and three RO and Milli Q systems (all purchased in 1987). We also request funds to purchase service contracts for our DNA sequencer BD LSR II FACS analyzer and the new cell sorter and confocal microscope requested in this proposal.