AREA IV IMMUNOLOGY

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。区域IV，免疫学的目标是了解控制免疫系统发育和功能的细胞与细胞相互作用和分子机制。在该组中，一个较小的亚组专注于在发育和调节水平上调节自身免疫的机制。第四区有六名成员，包括来自纽约市立大学医学院微生物学和免疫学系的2名研究活跃的教师和来自生物学系的4名教师。William Boto、Shubha Govind和Jerry Guyden是通过RCMI赠款直接聘用的。Mark Pezzano在担任RCMI项目副主任并与Guyden博士合作研究项目数年后，被招聘为生物系的教师职位。Paul Gottlieb和琳达Spatz被纽约市立大学医学院聘用，并被招募加入RCMI计划，因为他们在我们上次提案中发起免疫学小组时，积极开展了专注于自身免疫的研究合作。所有这些研究人员目前都有外部资金支持他们的研究项目，许多人在小组内部和外部研究人员都有合作项目。该小组的研究兴趣是多样化的，从果蝇的先天免疫系统发育和功能的研究到KSHV在人类中的传播和进化。我们有几个研究人员研究B和T细胞中免疫细胞发育和免疫系统调节的各个方面，这些方面集中在免疫学中的一个关键问题上，即免疫系统如何区分自我和非自我。这些研究与自身免疫性疾病的发展直接相关。在这个应用程序中，我们要求资金聘请一个额外的免疫学家，以增加我们在这个令人兴奋的研究领域的临界质量。这个人将有一个研究重点在脊椎动物的先天免疫，以最好地促进与我们的研究小组的现有成员的合作，并增加在近年来经历了新的兴趣革命领域的临界质量。 第四区的设备要求包括一台新的荧光激活细胞分选机，以取代在RCMI第一轮资金中购买的现已无法使用但使用率很高的Coulter Epics Elite。这种设备对于免疫学家的研究工作来说是不可或缺的，对于RCMI计划其他领域的许多研究人员来说也是非常重要的工具。 对于我们的中央设施，我们要求提供资金，以更换在第一个赠款期内购买的物品，这些物品不能再使用，供应商也不会继续为它们提供服务。我们需要更换两个用于中心设施设备室的寒潮冷却装置，以及一个高压灭菌器。我们需要增加两个<$80 Revco冰柜，以取代旧设备，并为新员工提供额外的存储空间。我们需要更换我们的分光光度计（1988年购买），一个speedvac，三个RO和Milli Q系统（都是1987年购买的）。我们还要求资金购买我们的DNA测序仪BD LSR II FACS分析仪和新的细胞分选仪和共聚焦显微镜在本提案中要求的服务合同。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of Area IV, Immunology, is to understand the cell-to-cell interactions and molecular mechanisms that control the development and function of the immune system. Within the group, a smaller subgroup is focused on the mechanisms that regulate autoimmunity, both at the developmental and regulatory levels. There are six members of Area IV, consisting of 2 research active faculty from the CUNY Medical School department of Microbiology and Immunology and 4 from the department of Biology. William Boto, Shubha Govind and Jerry Guyden were hired through the RCMI grant directly. Mark Pezzano was recruited for a faculty position in the Biology department, after serving as Deputy Director of the RCMI program and collaborating on research projects with Dr. Guyden for several years. Paul Gottlieb and Linda Spatz were hired by the CUNY Medical School and recruited to join the RCMI program because of their active research collaboration focused on autoimmunity when the immunology subgroup was initiated in our last proposal. All of these researchers currently have outside funding for their research projects and many have collaborative projects both within the group and with outside investigators. The research interests of the group are diverse, ranging from studies of innate immune system development and function in Drosophila to KSHV transmission and evolution in humans. We have several investigators who study aspects of immune cell development in both B and T cells and immune system regulation, which are focused on a key question in Immunology as to how the immune system distinguishes self from non-self. These studies are directly relevant to the development of autoimmune diseases. In this application, we are requesting funds to hire one additional immunologist to increase our critical mass in this exciting area of research. This individual will have a research focus in innate immunity in vertebrates, to best foster collaboration with the existing members of our research group and to increase the critical mass in an area that has experienced a revolution in new interest in recent years. The equipment requests for Area IV include a new fluorescence activated cell sorter, to replace the now non-functional but heavily utilized Coulter Epics Elite purchased in the initial round of RCMI funding. This equipment while indispensable to the research efforts of the Immunologists is also a very important tool for many researchers in other areas of the RCMI program. For our central facilities we are requesting funds for replacement of items purchased during the first granting period that can no longer be used nor will the vendors continue to service them. We need to replace two Cold wave chilling units, used for our central facility equipment rooms, as well as an autoclave. We need to add two ¿80 Revco freezers to replace old equipment and provide additional storage space for new hires. We need to replace our Spectrophotometer (purchased in 1988), a speedvac, and three RO and Milli Q systems (all purchased in 1987). We also request funds to purchase service contracts for our DNA sequencer BD LSR II FACS analyzer and the new cell sorter and confocal microscope requested in this proposal.