OPTICAL IMAGING FACILITY

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。作为先前资助的RCMI周期的一部分，1999年秋季在UCC建立了一个光学成像设施。该设施获得的第一个主要设备是一个灵敏的基于显微镜的系统，该系统允许以快速的时间分辨率评估细胞反应，如钙信号。该系统还允许对该功能变量进行空间评估，这意味着研究人员能够识别相邻细胞组中钙信号响应的异质性。这项提案要求提供资金，继续为这一过时的细胞成像系统提供技术支持，并维持其尖端设备。由于实验的性质，这种特殊的显微镜设置是一个高度专用的系统。因此，为了向其他基于显微镜的应用提供技术支持，本提案还要求提供资金，以支持将免疫细胞化学实验室纳入光学成像设施。免疫细胞化学实验室专门从事定性鉴定和定位的细胞轴承选择性标记，采用特异性抗体，这些分子。参考实验室是在Serguei Skatchkov和Misty Eaton博士的倡议下于1999年组织的，他们在其项目的实验设计中成功地使用了这项技术。1997年，Skatchkov博士在早期RCMI支持的方法学转移计划的帮助下，在柏林洪堡大学的一个领先的免疫细胞化学实验室接受了培训。 免疫细胞化学实验室设备包括一台直立荧光显微镜和一台高分辨率数码相机。在本实验室获得的免疫细胞化学数据已发表在同行评议的期刊上，并在地方和国家研讨会上发表。目前，有几个项目将直接受益于这种基于显微镜的技术提供的服务。因此，光学成像设备的目标是：1。为时间和空间分辨率成像系统提供技术支持.将免疫细胞化学实验室整合到光学成像设备中。在我们的学术界传播我们的设施的能力。继续支持光学成像设施将构成细胞和分子生物学中心的资产，因为它将有助于提高其现有参与者的生产力，并将构成潜在的新研究人员/合作者的有吸引力的研究资源。光学成像设施的免疫细胞化学能力将补充我们现有的成像平台，该平台被配置用于快速采集和分析细胞内钙的荧光标记报告分子。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. As part of a previous funded RCMI cycle, an Optical Imaging Facility was established at UCC during the fall of 1999. The first major equipment acquired for this facility was a sensitive microscope-based system that allows the assessment of cellular responses such as calcium signaling with fast temporal resolution. The system also allows the spatial assessment of this functional variable, which means that an investigator is able to identify the heterogeneity of calcium signal responses in neighboring groups of cells. This proposal requests funds to continue providing technical support for this time-lapsed cell imaging system and the maintenance of its sophisticated equipment. Due to the nature of experiments, this particular microscope setup is a highly dedicated system. Therefore, in order to provide technical support for other microscope-based applications, this proposal is also requesting funds to support the integration of an immunocytochemistry laboratory into the Optical Imaging Facility. The immunocytochemistry laboratory specializes in the qualitative identification and localization of cells bearing selective markers by employing specific antibodies to these molecules. The referenced laboratory was organized in 1999 through the initiatives of Drs. Serguei Skatchkov and Misty Eaton, who have been successfully using this technology in the experimental design of their projects. In 1997, Dr. Skatchkov, thanks to an earlier RCMI supported Methodology Transfer Program, received training in a leading immunocytochemistry laboratory at Humboldt University, Berlin. The immunocytochemistry laboratory equipment includes an upright fluorescence microscope and a high-resolution digital camera. Immunocytochemistry data obtained in this laboratory has been published in peer-reviewed journals and presented in local and national symposiums. Currently, there are several projects that will benefit directly from the service provided by this microscope-based technology. Therefore, the aims of the Optical Imaging Facility are:1. To provide technical support for the time and spatial resolution imaging systems.2. To integrate an immunocytochemistry laboratory into the Optical Imaging Facility.3. To disseminate in our academic community the capabilities of our facility.4. To work on the submission of a grant for a confocal microscope.The continued support of the Optical Imaging Facility will constitute an asset for the Cell and Molecular Biology Center since it will contribute to enhance the productivity of its current participants and will constitute an attractive research resource for potential new investigators/ collaborators. The immunocytochemisty capabilities at the Optical Imaging Facility will complement our existing imaging platform that is configured for the rapid acquisition and analysis of fluorescently tagged reporters of intracellular calcium.