ROLE OF CHEMOKINES IN MUCOSAL IMMUNITY: ORAL, RESPIRATORY & UROGENITAL PATHOGENS

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。本项目的第一个目的是评估粘膜和全身免疫缺陷的小鼠在产生或识别趋化因子后，粘膜免疫与Th 1或Th 2诱导方案。第二个目标是确定这些趋化因子用于增强获得性免疫的G蛋白依赖性和非依赖性机制。第三个目标将评估CD 28-B7和CD 40-CD 40 L相互作用以及B和T细胞分化对趋化因子介导的适应性免疫中发生的抗体、T辅助细胞因子和细胞毒性T细胞应答的作用。第四个目标将定义当趋化因子与蛋白抗原通过粘膜或全身途径给药时产生的Th 1和Th 2型细胞因子依赖性和非依赖性途径。拟议的研究将提供重要的和新的信息有关的细胞机制，趋化因子，RANTES，Lptn，IP-10和IL-8用于诱导和调节全身和粘膜免疫。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The first aim of this project will assess the mucosal and systemic immunity in mice defective in the production or recognition of chemokines following mucosal immunization with Th1- or Th2-inducing regimens. The second aim will determine the G protein(s) - dependent and -independent mechanisms that these chemokines use to enhance acquired immunity. The third aim will assess the role of CD28-B7 and CD40-CD40L interactions as well as B and T cell differentiation for antibody, T helper cytokine, and cytotoxic T cell responses that occur in chemokine-mediated adaptive immunity. The fourth aim will define the Th1- and Th2-type cytokine -dependent and -independent pathways that result when chemokines are administered with protein antigen through mucosal or systemic routes. The proposed studies will provide important and novel information regarding the cellular mechanisms that chemokines, RANTES, Lptn, IP-10, and IL-8 use to induce and regulate systemic and mucosal immunity.