Development of an ex vivo buccal mucosal model for understanding the biology of oral health

在本项目中，建议收集猪口腔粘膜组织作为食品工业的副产品，并以与皮肤相似的方式，开发一种稳健的离体口腔粘膜模型1 -3，该模型在较长时间内保持健康的口腔组织，适用于研究口腔保健材料。这里要采取的方法将涉及：（i）收集猪口腔粘膜组织（ii）鉴定合适的培养基以维持组织的活力（iii）确认模型的屏障功能特性（iv）评估炎症和氧化应激的组织生物标志物。从长远来看，该模型应该允许研究口腔组织中炎症和氧化应激的生物标志物，以及对口腔粘膜腔如何响应特定制剂的应用的新见解。最终，该项目应提供有关促进和维持屏障功能和支持口腔健康的战略的新信息。 该项目将建立新的口腔上皮模型，用于快速开发和筛选口腔健康管理配方，并将引起学术界和工业界的极大兴趣。

In this project it is proposed to collect porcine oral mucosal tissue as a by-product of the food industry and in a similar manner as for skin, develop a robust ex vivo oral mucosal model1-3 that maintains healthy buccal tissue over an extended period suitable for investigating oral healthcare materials. The approach to be taken here will involve: (i) Collection of porcine oral mucosal tissue (ii) Identification of suitable media to maintain the viability of the tissue (iii) Confirmation of barrier function properties of the model (iv) Assessment of tissue biomarkers of inflammation and oxidative stress. In the longer term the model should allow the study of biomarkers of inflammation and oxidative stress in oral tissues and new insights into how the oral mucosal cavity responds to application of specific formulations. Ultimately the project should delivery new information concerning strategies to promote and maintain barrier function and support oral health. This project will establish new models of the oral epithelium for rapid development and screening of formulations for management of oral health and will be of considerable interest to academia and industry.