Characterising the genetic control of quality attributes in Pak Choi Brassica rapa subsp. Chinensis (L.).

1.集合资源的分子定义CN种子有大量的白菜种质用于其育种计划。对于这个新项目，我们将用组装的B来补充这些资源。在JIC和瓦赫宁根大学举行的rapa/ Pak Choi核心集合和公开的双亲群体。该ICASE奖项的第一阶段将采用高通量基因型测序技术，以生成与最新B对齐的高密度基因型数据。芜菁基因组组装这些数据将用于探索种群的子结构和个体之间的关系，建立一个新的基于遗传学的遗传资源描述。单倍型将在新的和现有的种质中定义，提供一种跟踪渗入片段的方法，加快先进回交系的创建，并提供一种在公司组合中定义知识产权的方法。2.开发用于增强育种的管道组装的种质将被表征为对品种开发和感官质量至关重要的形态性状，例如，农艺性状：植株形态、抽薹习性（开花时间）、茎开裂（基部）、柔软度;叶质量：颜色、萎蔫;收获后叶黄化。这些性状的平均表型数据将与新的GBS SNP数据相结合，以剖析这些性状的遗传调控，定义可用的等位基因变异。这些数据将使CN Seeds能够有效地选择种质，并建立一个新的分子育种平台。

1. A molecular definition of assembled resourcesCN Seeds have a large collection of Pak Choi germplasm used in their breeding programme. For this new project, we will supplement these resources with assembled B. rapa/ Pak Choi core collections and publicly available bi-parental populations held at JIC and Wageningen University. The first phase of this ICASE award will employ high-throughput Genotype-By-Sequencing to generate high-density genotype data aligned with the latest B. rapa genome assembly. These data will be used to explore population sub-structure and relationships between individuals, to establish a new phylogeny-based description of the genetic resources. Haplotypes will be defined across new and existing germplasm, providing a means to track introgressed segments, speed up creation of advanced backcross lines, and provide a means to define intellectual property within the company's portfolio.2. Developing a pipeline for enhanced breedingThe assembled germplasm will be characterised for morphological traits key to varietal development and organoleptic quality, e.g., agronomic characters: plant shape, bolting habit (flowering time), stem cracking (base), suppleness; leaf quality: colour, wilting; post-harvest leaf yellowing. Mean phenotype data for these traits will be combined with new GBS SNP data to dissect the genetic regulation of these traits, define available allelic variation. These data will enable the efficient selection of germplasm and establish a new molecular breeding platform at CN Seeds.