Core--Animal Models and Pathology

动物模型和病理学设施核心的总体目标是为中心成员提供最先进的病理学支持，转基因资源和中央管理的AAALAC批准的动物饲养和手术设施。该中心配备了经验丰富的工作人员，并配备了必要的设备，以产生基因工程小鼠（GEM），通过胚胎移植再衍生进口小鼠，提供集落管理，并通过组织学和图像分析制备和解释组织样本。具体目标如下： (1)通过原核显微注射DNA构建体或胚胎细胞来产生转基因动物 干细胞操作和提供GEM集落管理供中心成员使用; (2)通过胚胎移植重新获得转基因和其他专门的小鼠品系，以确保本计划中使用的所有小鼠均无特定病原体; (3)提供组织学、病理学、分子表征和影像学专业知识，用于GEM和其他动物模型的广泛表型分析和病理损伤评估。

The overall objective of the Animal Models and Pathology Facilities Core is to provide Center members with state-of-the-art pathology support, transgenic resources and a centrally-managed AAALAC-approved animal holding and surgical facility. The Core is staffed with experienced personnel and is equipped with essential equipment to generate genetically engineered mice (GEM), rederive imported mice by embryo transfer rederivation, provide colony management, and prepare and interpret tissue samples by histological and image analysis. The specific aims are as follows: (1) To generate transgenic animals by pronuclear microinjection of DNA constructs or embryonic stem cell manipulation and provide colony management of GEM for use by Center members; (2) To rederive transgenic and other specialized mouse strains by embryo transfer to assure specific pathogen free status of all mice used in this program; (3) To provide histology, pathology, molecular characterization and imaging expertise for extensive phenotyping and assessment of pathological damage of GEM and other animal models as needed.