CORE F: MORPHOLOGY AND IMAGING

形态和成像核心的总体目标是通过继续提供CURE：DDRCC研究人员来提高CURE：DDRCC计划的有效性，他们拥有独立资助的研究项目，拥有设施、培训和援助，对天然和转基因细胞中的递质、受体、通道和其他整合蛋白质进行形态、成像和功能研究。核心继续提供可视化细胞和组织的组织和功能的服务。这些方法包括一系列在细胞生物学和神经生物学中有广泛应用的当代形态和成像方法，并将增加新的或改进的成像技术，以促进对 在过去几年中发展起来的信号转导。此外，动物病理学服务将可用于确定可能的表型变化和病理条件，这些可能与大多数治愈方法越来越多地使用的基因操纵小鼠有关：DDRCC调查人员。此外，还将通过Core使用机构内的非侵入性神经成像设施，以满足对研究与压力相关的病理中的大脑变化的日益增长的技术需求。该中心将提供专业知识和技术支持，并将协调加州大学洛杉矶分校和退伍军人管理局大洛杉矶医疗保健系统可用的成像资源。核心将会 协商对这些资源的访问并协调其使用，确保成像会话的时段安全，根据需要提供技术人员支持，提供对用于图像归档、数据分析和生理和统计建模的集中计算资源的访问，并为研究设计和数据分析提供咨询服务。以下服务将包括：免疫组织化学、光学显微镜和数字摄影的援助和培训、计算机辅助图像处理和分析、共聚焦显微镜、钙离子成像、用于体外测量细胞内cAMP水平的荧光成像系统、动物病理学服务，以及使用非侵入性神经成像方法的设施。

The overall goal of the Morphology and Imaging Core is to enhance the effectiveness of the CURE: DDRCC program by continuing to provide CURE: DDRCC investigators who have independently-funded research projects with facilities, training and assistance for morphological, imaging and functional studies of transmitters, receptors, channels and other integral proteins in native and transfected cells. The Core continues to offer services for visualizing cellular and tissue organization and function. These include an array of contemporary morphologic and imaging approaches that have a broad application in cell biology and neurobiology and will add new or improved imaging technologies to facilitate investigations in signal transduction that have developed during the past several years. In addition, animal pathology service will be available to determine possible phenotypical alterations and pathological conditions that may be associated with genetically manipulated mice that are increasingly used by most CURE: DDRCC investigators. Furthermore, access to facilities within the institution for non-invasive neuroimaging will also be available through the Core for the increasing demand of technologies to investigate brain changes in stress-related pathologies. The Core will supply expertise and technical support, and will coordinate the imaging resources available at UCLA and the VA Greater Los Angeles Healthcare System. The core will negotiate access to these resources and coordinate their utilization, secure slots for imaging sessions, provide technologist support as needed, offer access to centralized computational resources for image archiving, data analysis and physiological and statistical modeling, and provide consulting services for study design and data analysis. The following services will be included: Immunohistochemistry, Assistance and training for Light Microscopy and Digital Photography, Computer-assisted image processing and analysis, Confocal microscopy, Ca2+ imaging, Fluorescent imaging system for in vitro measurement of intracellular cAMP levels, Animal pathology service, and Access to facilities for non-invasive neuroimaging approaches.