A PCT Sample Preparation System for Proteomic Research and Clinical Diagnostics

项目概述/摘要：预计在不久的将来，通过蛋白质组学研究的进步，公共卫生将得到实质性的改善，从而改善人类疾病的诊断。这些进步将需要新的工具和标准化方法来进行基础和应用蛋白质组学研究以及临床诊断。目前，用于蛋白质组学和临床诊断的样品制备系统和方法缺乏必要的效率、准确性或稳健性，无法充分阐明蛋白质组学，从而有效地预测疾病的易感性或对药物治疗的反应。在此，我们建议基于Pressure BioSciences的压力循环技术（PCT）开发一种改进的系统和标准方法来研究蛋白质组，PCT是一种从细胞和组织中提取分子的强大技术。本研究的里程碑包括：1)从研究和临床相关样品中提取蛋白质，以证明安全、可重复（准确）和高质量蛋白质提取的可行性；2)提取高质量和定量的生物标志物；3)提取分子复合物，如细胞器。我们的最终目标是开发和建立一个基于压力循环技术（PCT）的蛋白质提取标准方法的综合系统，以加速蛋白质组学的发现。项目描述：与人类基因组计划相对应，蛋白质组学的研究和应用开发引领了新的集中努力，旨在通过揭示人类蛋白质组及其与人类疾病的相关性来改善公共卫生。由于人类蛋白质组非常复杂，需要改进、可靠和敏感的技术来进行数据生成和分析。本课题旨在开发压力循环技术，为蛋白质组学研究和临床诊断提供高质量、高数量的蛋白质提取技术，对加快蛋白质组学研究和临床应用开发具有重要意义。

DESCRIPTION (provided by applicant): Project Summary/Abstract: Substantial improvements in public health are anticipated in the near future through advancements in proteomic research leading to improvements in diagnosis of human diseases. These advancements will require new tools and standardized methods for basic and applied proteomic research, and clinical diagnostics. At this time, sample preparation systems and methods being used in proteomics and clinical diagnostics, lack the necessary efficiency, accuracy or robustness to adequately elucidate the proteome such that prediction of susceptibility to disease or response to drug therapy is efficacious. Here, we propose to develop an improved system and standard methods to study the proteome based on Pressure BioSciences' Pressure Cycling Technology (PCT), a proven powerful technology in extracting molecules from cells and tissues. Milestones of this proposed study include 1) Extraction of proteins from research and clinically relevant samples to demonstrate the feasibility of safe, reproducible (accurate) and high quality protein extraction; 2) Extraction of high quality and quantitative biomarkers, and 3) Extraction of molecular complexes, such as organelles. Our ultimate goal is to develop and establish a comprehensive system with standard methods for the extraction of proteins based on Pressure Cycling Technology (PCT) to accelerate discoveries in proteomics. Project Narrative: Mirrored to human genome project, proteomics research and application development leads new concentrated efforts aiming to improve public health by revealing human proteome and its correlation with human diseases. Since human proteome is very complex, improved, reliable, and sensitive technologies are required for data generation and analysis. This proposed study aims to develop Pressure Cycling Technology for extracting high quality and quantity of proteins for the proteomic research and clinical diagnostics that may be highly significant in accelerating proteomic research and clinical application development.