Label-Free NanOFET Microarrays with Embedded-Probe Nanocomposite Active Layers

描述(由申请人提供)：Nanohmics，Inc.和Ambion，Inc.与加州大学戴维斯分校的Susan Kauzlarich博士和德克萨斯大学奥斯汀分校的Lynn Loo博士合作，提议开发一种用于紧凑、无标记检测寡核苷酸分析的纳米复合有机场效应晶体管(NanOFET)多路微阵列平台。该方法将包括开发由掺杂纳米颗粒和导电聚合物组成的超薄、高载流子迁移率的聚合物/半导体复合材料。所提出的几何结构提供了一种测量电荷效应和杂交反应引起的局域温度变化的方法。核苷酸锚定将被固定在纳米颗粒的表面，并嵌入到复合活性层中。这些有序的复合有源层将使用单步转移技术在NanOFET阵列的整个表面上沉积。光刻图案化的源极和漏极触点将作为每个探针寡核苷酸在文库中延伸的独立固定位置。拟议研究的具体目标是开发一种不需要二次标记步骤的高通量分析平台。在这一点上，具有多个目标的微阵列分析可以用单个样品等份进行，并可以使用紧凑的读取器设备进行分析。所提出的方法将首次尝试将寡光耦合化学与场效应晶体管阵列相结合，以制备具有嵌入探针锚的有源结构。具有所提出的新型有源半导体层的FET几何结构的开发将提供一种用于将FET阵列缩放到生产微阵列器件的手段，而不是具有有限可扩展性的其他几何结构。微阵列将作为无标记的直接电转导平台运行，并通过紧凑型读取器仪器进行分析，包括现场应用，在这些应用中，可能对寡核苷酸样本进行实时疾病状态、病原体或生物安全检测。

DESCRIPTION (provided by applicant): Nanohmics, Inc. and Ambion, Inc. working in consortium with Dr. Susan Kauzlarich at the University of California Davis and Dr. Lynn Loo at the University of Texas at Austin propose to develop a Nanocomposite Organic Field-Effect Transistor (NanOFET) multiplex microarray platform for compact, label-free detection of oligonucleotide assays. The proposed method will involve the development of ultrathin, high carrier mobility, polymeric/semiconductor composites consisting of doped nanoparticles and conducting polymers. The proposed geometry provides a means to measuring both charge effects and localized temperature change resulting from the hybridization reaction. Nucleotide anchors will be immobilized to the surface of the nanoparticles and be embedded in the composite active layer. These organized composite active layers will be deposited using a single step transfer technique over the entire surface of the NanOFET array. Lithographically patterned source and drain contacts will serve as independent immobilization sites for extension of each probe oligonucleotide in the library. The specific aim of the proposed research is to develop a high throughput analysis platform that does not require a secondary labeling step. In this regard, microarray analysis with multiple targets can be performed with a single sample aliquot and be analyzed using compact reader devices. The proposed method will involve the first attempt to integrate oligo photocoupling chemistry with a field effect transistor array in the preparation of active structures with embedded probe anchors. The development of FET geometry with the proposed novel active semiconducting layer will provide a means for scaling FET arrays to production microarray devices as opposed to other geometries that have limited scalability. The microarray will operate as a label-free, direct electrical transduction platform and be analyzed by compact reader instrumentation, including field applications, where it may be possible to perform real time disease state, pathogen or biothreat detection on oligonucleotide samples.