Multi-spectral Mosaic (MSM) Digital Imaging Sensor for Microscopy Applications

描述（由申请人提供）：联合收割机了高空间和光谱分辨率优点的紧凑、低成本数字成像系统是生物分析分析的需求，如原位杂交（ISH）和荧光原位杂交（FISH）核型分析、组织和细胞诊断以及微阵列。商业图像传感器的进步已经导致提供精细空间分辨率的像素密度。尽管像素密度足以分辨具有大视场的超细特征，但商业上可用的彩色CMOS相机在光谱（色度）分辨率的水平上仍然受到限制。彩色成像系统通常受到拜耳滤色器阵列（CFA）马赛克图案化（即，通常是RGB三色滤色器阵列（CFA））的限制。这些彩色相机芯片旨在满足数码摄影的最低色彩再现要求。通过单色图像传感器与体积庞大且昂贵的色散/滤光器元件的组合实现了更高的光谱带清晰度，特别是对于涉及滤光器组之间的电动切换的那些。同样地，电子可调谐滤波器（例如LCD、声光、Sagnac等）慢图像获取速度是不理想的。消除外部色散元件和缓慢的可调谐滤波器需要直接将更高的光谱清晰度集成到图像传感器（CMOS/CCD）上的马赛克图案中。Nanohohmos与Atactic Technologies合作，提出开发一种直接在CMOS传感器表面上扩展彩色滤光片阵列（CFA）的光谱分辨率和范围（例如UV，可见光和近红外）的工艺。此外，这些多光谱镶嵌（MSM）图案将使用一种新的方法沉积，该方法利用寡核苷酸微阵列的锁和钥匙配准，以将核心染色的微球的表面固定化到图像传感器上的镶嵌图案中。所提出的方法是大大不同于标准的串行光刻处理目前使用的商业准备现有的彩色相机CFA。所提出的寡核苷酸定向固定将导致在单个处理步骤中形成整个镶嵌图案。该技术是利用生物分子识别技术对材料层进行生物工程改造，并应用于商业设备中。 紧凑型图像传感器的光谱能力的扩展将使得能够在包括微阵列分析、诊断和核型分析的许多领域中对生物分子过程进行更快速和成本有效的分析。此外，增强的光谱分辨率将导致所有图像传感器应用的更高的色彩保真度再现。使用生物分子材料进行生物工程是拟议研究的一个重要特征

DESCRIPTION (provided by applicant): Compact, low-cost digital imaging systems that combine the benefits of high spatial and spectral resolution are in demand for bioanalytical analyses such as in situ hybridization (ISH) and fluorescence in situ hybridization (FISH) karyotyping, tissue and cellular diagnostics, and microarrays. Advances in commercial image sensors have led to pixel densities which provide exquisite spatial resolution. Despite pixel densities sufficient to resolve ultrafine features with large fields of view, commercially available color CMOS cameras are still limited in the level of spectroscopic (chromaticity) resolution. Color imaging systems are generally restricted by the Bayer color filter array (CFA) mosaic patterning (i.e. usually RGB three color filter arrays (CFAs). These color camera chips are designed to meet minimal color reproduction requirements for digital photography. Higher spectroscopic band definition is achieved through combination of a monochrome image sensor with dispersive/filter elements that are bulky and expensive, particularly for those involving motorized switching between filter sets. Likewise, electronically tunable filters (e.g. LCD, acousto-optic, Sagnac, etc.) that slow image acquisition speed are not ideal. Elimination of external dispersive elements and slow tunable filters requires directly integrating higher spectral definition into the mosaic pattern on the image sensor (CMOS/CCD). Nanohmics, in collaboration with Atactic Technologies, propose to develop a process for expanding the spectral resolution and range (e.g. UV, visible and near-IR) of Color Filter Arrays (CFAs) directly on the CMOS sensor surface. Furthermore, these Multi-Spectral Mosaic (MSM) patterns will be deposited using a novel method that utilizes the lock-and- key registration of oligonucleotide micorarrays to direct surface immobilization of core- dyed microspheres into the mosaic pattern on the image sensor. The proposed method is vastly different than standard serial lithographic processing currently used in commercial preparation of existing color camera CFAs. The proposed oligo-directed immobilization will lead to the development of the entire mosaic pattern in a single processing step. This proposed technique was exploit biomolecular recognition to bioengineer the material layer in a commercial device application.Project Narrative The expansion of spectral capabilities of a compact image sensor would enable more rapid and cost effective analyses of biomolecular processes in a number of fields including microarray analysis, diagnostics, and karyotyping. Furthermore, enhanced spectral resolution will lead to higher color fidelity reproduction for all image sensor applications. The use of biomolecular materials for bioengineering is an important enabled feature of the proposed research