A Turnkey, Wireless, EEG/EMG/Biosensor Measurement

描述（由申请人提供）：该项目的长期目标是为大鼠和小鼠开发，验证和商业化无线，头戴式，交钥匙，带有集成生物传感器的EEG/EMG系统。进一步的目标是开发76 μ m葡萄糖生物传感器，用于直接测量大鼠和小鼠的脑葡萄糖水平。第一阶段项目的具体目标是开发和测试76 μ m葡萄糖生物传感器，用于小鼠的系留脑电图/肌电图/生物传感器解决方案以及用于大鼠的无线解决方案。为了实现这些目标，Pinnacle Technology、西北大学和堪萨斯大学正在利用过去在谷氨酸生物传感器、大鼠无线电势器和小鼠拴系脑电图/肌电图系统设计方面的成功经验。这些产品是在单独的合作中开发的，目前正在商业化。即将推出的产品包括：用于小鼠的系绳系统，用于大鼠的无线系统，以及最终用于小鼠的完全无线系统。商业应用包括睡眠研究、行为研究和药物筛选。技术创新包括生物传感器设计、交钥匙头戴式设计、先进的电子设计和先进的低功率射频设计。在记录啮齿动物睡眠的同时，测量体内特定脑区葡萄糖的能力将使研究人员能够更好地检查睡眠过程中大脑特定部位的功能，并利用使用大鼠和小鼠模型进行研究所带来的优势。目前，在小鼠模型中同时研究睡眠和葡萄糖调节是不可能的，只有一篇已发表的文章在大鼠身上进行了尝试。即时记录睡眠小鼠或大鼠体内的葡萄糖水平，并将其活动与脑电图/肌电图相关联的能力，对研究睡眠和新陈代谢的研究人员来说将是有价值的。在小鼠模型中对葡萄糖的研究将为研究诸如NIRKO小鼠（脑/神经元特异性胰岛素受体敲除）等物种的基因突变开辟新的途径，这些基因突变可能会改变睡眠时的葡萄糖反应，并为睡眠和新陈代谢之间的联系提供线索。

DESCRIPTION (provided by applicant): The long term objective of this project is to develop, validate and commercialize wireless, head mounted, turnkey, EEG/EMG systems with an integrated biosensor for rats and mice. A further objective is to develop a 76 uM glucose biosensor for direct measurement of brain glucose levels in rats and mice. The specific aims of this Phase I project are to develop and test a 76 uM glucose biosensor, a tethered EEG/EMG/Biosensor solution for mice and a wireless solution for rats. To reach these objectives, Pinnacle Technology, Northwestern University and the University of Kansas are building on past successes in the design of glutamate biosensors, wireless potentiostats for rats, and tethered EEG/EMG systems for mice. These products were developed in separate collaborations and are currently being commercialized. Products to be introduced include: a tethered system for mice, a wireless system for rats, and ultimately a fully wireless system for mice. Commercial applications include sleep research, behavioral research and drug screening. Technological innovations include biosensor design, turnkey head mount design, advanced electronics design, and advanced low power radio frequency design. The ability to measure glucose from specific brain areas in vivo while simultaneously recording sleep in rodents will give researchers the ability to better examine the functioning of specific sites within the brain during the sleep process as well as leverage the advantages conferred by using rat and mouse models for research. At the moment, it is not possible to concurrently study sleep and glucose regulation in a mouse model and there is only one published account where it has been attempted in the rat. The ability to instantly record glucose levels in a sleeping mouse or rat, and correlate that activity with EEG/EMG, will be valuable to researchers studying sleep and metabolism. The investigation of glucose in a mouse model will open up new avenues of research with genetic mutations available in species such as the NIRKO mouse (brain/neuron-specific insulin receptor knockout) which may have altered glucose responses during sleep and provide clues as to how sleep and metabolism are linked.