Modelling host/parasitic nematode interactions with ovine 'mini-gut' organoids.

绵羊成体干细胞可以在实验室中用来生长3D胃肠道(GI)结构，称为GI有机体。在我们的实验室中，我们已经开发出绵羊的迷你肠道GI有机化合物，可以研究宿主和寄生胃肠道线虫(GIN)之间的相互作用。在温带地区，棕色胃虫Teladorsagia Circincta是绵羊的主要杜松子酒。这种寄生虫在皱胃(类似于胃部)中生长，造成与宿主炎症免疫反应相关的显著的粘膜损伤。为了确定感染，圆锥虫分泌排泄/分泌(ES)产物，用来调节宿主的免疫反应和细胞生理。越来越多的证据表明，胞外囊泡(EVS)是ES的一个亚成分，在宿主/寄生虫的相互作用中起着重要的作用。本项目的总体目标是利用绵羊皱胃有机化合物来鉴定和表征环纹夜蛾EVS的活性成分，并研究它们对寄主生理的影响。

Sheep adult stem cells can be used in the lab to grow 3D gastrointestinal (GI) structures called GI organoids. In our lab we have developed sheep 'mini-gut' GI organoids that allow the study of interactions between the host and parasitic gastrointestinal nematodes (GINs). In temperate regions the brown stomach worm Teladorsagia circumcincta is the principal GIN of sheep. This parasite develops in the abomasum (which is analogous to the gastric stomach) causing significant mucosal damage associated with host inflammatory immune responses. To establish infection T. circumcincta secretes excretory/secretory (ES) products that it uses to modulate the immune response and cellular physiology of the host. There is emerging evidence that extracellular vesicles (EVs), a subcomponent of ES, plays an important role in host/parasite interactions. The overall aim of this project is to use ovine abomasum organoids to identify and characterise active components of T. circumcincta EVs and to study their impacts on host physiology.