MOLECULAR EPIDEMIOLOGY OF STLV-I IN WILD AND CAPTIVE SOOTY MANGABEYS

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。研究了在杜兰国家灵长类动物研究中心(TNPRC)长期建立的黑猩猩(SM；Cercocebus Atys)群体中猴T淋巴细胞嗜淋巴病毒(STLV)的流行率和多样性。血清学分析确定39只动物中有22只(56%)STLV-1阳性。第二组来自非洲塞拉利昂的13个SM丛林肉样本也被包括在内，并仅通过PCR进行测试。39只圈养动物中有22只(56%)和13份丛林肉样品中有3份(23%)呈STLV-1阳性。核苷酸测序和系统发育分析表明，来自TNPRC群体和塞拉利昂的STLV-ISM毒株与先前报道的Yerkes国家灵长类动物研究中心(YNPRC)的STLV-1sm毒株形成了一个单一的簇。这些数据证实了TNPRC STLV-1sm的非洲来源，并表明美国的SM群体来自塞拉利昂。TNPRC STLV-1sm毒株进一步分为两个亚群，表明两个原始创始人Sms在输入美国时感染了STLV-1sm。TNPRC菌落中STLV-1sm的多样性与已报道的SIVsm菌落的高度多样性相匹配。TNPRC株的SIVsm谱系与STLV-1sm亚群分布之间缺乏相关性，提示两种病毒的克隆内传播是独立的事件。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A study was conducted to evaluate the prevalence and diversity of simian T cell lymphotropic virus (STLV) within the long established Tulane National Primate Research Center (TNPRC) colony of sooty mangabeys (SM; Cercocebus atys). Serological analysis determined that 22 of 39 animals (56%) were positive for STLV-1. A second group of thirteen SM bush-meat samples from Sierra Leone, Africa was also included and tested only by PCR. Twenty-two of 39 captive animals (56%) and 3 of 13 bush-meat samples (23%) were positive for STLV-1 by PCR. Nucleotide sequencing and phylogenetic analysis of viral strains obtained demonstrated that STLV-Ism strains from the TNPRC colony and Sierra Leone formed a single cluster together with the previously reported STLV-1sm strain from Yerkes National Primate Research Center (YNPRC).These data confirm an African origin for TNPRC STLV-1sm and suggest a Sierra Leone origin for the SM colonies in the United States. The TNPRC STLV-1sm strains further divided into two subclusters suggesting STLV-1sm infection of two original founder SMs at the time of their importation into the United States. STLV-1sm diversity in the TNPRC colony matches the already reported colony's high diversity of SIVsm. The lack of correlation between SIVsm lineage and STLV-1sm subcluster distribution of TNPRC strains suggest that intracolony transmission of both viruses were independent events.