Mechanisms of Olfactory Interneurnon Differentiation

描述(申请人提供)：本提案的目的是描述来源于脑室下区干细胞龛的哺乳动物成年出生的嗅球中间神经元的基本特征和作用。为了确定早期出生的中间神经元是否不同于成年产生的中间神经元，以及确定活动对成人来源的中间神经元分化的影响，已经开发了两个特定的目标。具体目的1：为了确定胚胎和围产期神经元发育过程中分化的嗅球中间神经元是否与成年神经元前体分化的神经元不同，将进行遗传命运图谱实验。具体目的2：为了表征活动对成人来源的嗅球中间神经元的影响，将在基因消融活动后评估中间神经元的形态、基因表达和存活。这些实验将提供有关成年神经发生对成熟动物功能的意义和贡献的重要信息。此外，有关成人神经元分化机制的信息将有助于引导这种分化朝着疾病或损伤后的神经系统修复方向发展。

DESCRIPTION (provided by applicant): The objective of this proposal is to characterize the essential characteristics and role of mammalian, adult-born olfactory bulb interneurons derived from the subventricular zone stem cell niche. Two specific aims have been developed to determine if early-born interneurons differ from those generated in the adult, and to determine the influence of activity on the differentiation of adult-derived interneurons. Specific Aim 1: In order to determine whether olfactory bulb interneurons differentiating during embryonic and perinatal neuronal development are different from those derived from adult neuronal precursors, genetic fate-mapping experiments will be performed. Specific Aim 2: To characterize the influence of activity on adult-derived olfactory bulb interneurons, interneuron morphology, gene expression, and survival will be assessed following genetic ablation of activity. These experiments will contribute important information regarding the significance and contribution of adult neurogenesis to the function of the mature animal. In addition, information regarding the mechanism of neuronal differentiation in the adult will aid in efforts to guide that differentiation toward nervous system repair following disease or injury.